Cytotoxicity Evaluation of Two Bis-Acryl Composite Resins Using Human Gingival Fibroblasts
| Autor: | Marco Antonio Gallito, Felipe Leite de Oliveira, Miriam F. Zaccaro Scelza, Gutemberg Gomes Alves, Fabiano Palmeira Gonçalves, Vladi Oliveira Guimarães Júnior |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Neutral red
Biocompatibility Composite number Gingiva 02 engineering and technology Composite Resins 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine biocompatibility fibroblasts Humans Viability assay Crystal violet Primary cell Composite material Cytotoxicity General Dentistry Chromatography Chemistry 030206 dentistry temporary dental restoration Fibroblasts 021001 nanoscience & nanotechnology Cell culture 0210 nano-technology |
| Zdroj: | Brazilian Dental Journal, Volume: 27, Issue: 5, Pages: 492-496, Published: OCT 2016 Brazilian Dental Journal v.27 n.5 2016 Brazilian Dental Journal Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
| Popis: | Bis-acryl resins are used for temporary dental restorations and have shown advantages over other materials. The aim of this work was to evaluate the in vitro cytotoxicity of two bis-acryl composite resins (Protemp 4 and Luxatemp Star), obtained at 1, 7 and 40 days after mixing the resin components, using a standardized assay employing human primary cells closely related to oral tissues. Human gingival fibroblast cell cultures were exposed for 24 h to either bis-acryl composite resins, polystyrene beads (negative control) and latex (positive control) extracts obtained after incubation by the different periods, at 37 °C under 5% CO2. Cell viability was evaluated using a multiparametric procedure involving sequential assessment (using the same cells) of mitochondrial activity (XTT assay), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). The cells exposed to the resin extracts showed cell viability indexes exceeding 75% after 24 h. Even when cells were exposed to extracts prepared with longer conditioning times, the bis-acryl composite resins showed no significant cytotoxic effects (p>0.05), compared to the control group or in relation to the first 24 h of contact with the products. There were no differences among the results obtained for the bis-acryl composite resins evaluated 24 h, 7 days and 40 days after mixing. It may be concluded that the bis-acryl resins Protemp 4 and Luxatemp Star were cytocompatible with human gingival fibroblasts, suggesting that both materials are suitable for use in contact with human tissues. Resumo Resinas bisacrílicas são usadas em restaurações dentárias provisórias e têm mostrado vantagens em relação a outros materiais. O objetivo deste trabalho foi avaliar a citotoxicidade in vitro de duas resinas compostas bisacrílicas (Protemp 4 e Luxatemp Star), obtidas após 1, 7 e 40 dias da mistura com os componentes da resina, usando um ensaio padronizado empregando células primárias humanas fortemente relacionadas aos tecidos orais. Culturas de células de fibroblastos gengivais humanos foram expostas por 24 h aos extratos das resinas bisacrílicas, esferas de poliestireno (controle negativo) e látex (controle positivo), obtidos após diferentes períodos de incubação, a 37 °C e com 5% CO2. A viabilidade celular foi avaliada usando procedimentos multiparamétricos que envolvem a avaliação sequencial (usando as mesmas células) da atividade mitocondrial (ensaio XTT), a integridade de membrana (teste do vermelho neutro) e a densidade celular total (teste de exclusão do corante cristal violeta). As células expostas aos extratos mostraram viabilidade celular acima de 75% depois de 24 h. Mesmo quando as células foram expostas aos extratos com aumento do tempo de condicionamento, as resinas bisacrílicas não apresentaram efeitos citotóxicos significativos (p |
| Databáze: | OpenAIRE |
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