Effect of electrical discharging on formation of nanoporous biocompatible layer on Ti-6Al-4V alloys
Autor: | Mao Suan Huang, Pei Yi Wang Wang, Tzu Sen Yang, Meng Yuan Tsai, Mao Sheng Wang, Ming-Hong Lin |
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Rok vydání: | 2013 |
Předmět: |
Anatase
Materials science Surface Properties Osteocalcin Cell Culture Techniques Cell Line Crystallinity X-ray photoelectron spectroscopy Coated Materials Biocompatible Materials Testing Alloys Cell Adhesion Humans Cell Shape Microscale chemistry Cell Proliferation Titanium Crystallography Osteoblasts Nanoporous Photoelectron Spectroscopy Cell Differentiation Adhesion Electrochemical Techniques Alkaline Phosphatase Nanostructures Oxygen Chemical engineering Microscopy Electron Scanning Wettability Surface modification Oral Surgery Layer (electronics) Porosity Dental Alloys |
Zdroj: | Implant dentistry. 22(4) |
ISSN: | 1538-2982 |
Popis: | PURPOSE In this study, the electrical discharge machining (EDM) was formed on the surface of the Ti-6Al-4V (Ti64) specimen. MATERIALS The properties of adhesion and proliferation of MG-63 cells were evaluated the interactions between the EDM-treated layer and cells. RESULTS The incorporation of oxygen roughened the EDM-treated specimen surface on a microscale, where the nanoscale pores were superimposed. The EDM-treated layer, which can generate the thick anatase TiO2 on the Ti64 surface, afforded a cytocompatible environment. In cell culture, alkaline phosphatase activity and osteocalcin can be dramatically enhanced on the EDM-treated surfaces when compared with the untreated surface. In addition, the increase in peak currents to the EDM functionalization led to enhancement of multiple osteoblast functions. CONCLUSIONS This study reveals that the chemistry and crystallinity of the EDM-treated layer played important roles in affecting osteoblastic responses to the specimens, which provided insight into the development of new biomedical implant surfaces. |
Databáze: | OpenAIRE |
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