Highly sensitive electrochemical detection of the marine toxins okadaic acid and domoic acid with carbon black modified screen printed electrodes
| Autor: | Lea Mühlebach, Silvia Generelli, Linda D. Stewart, Katrina Campbell, Davide Migliorelli, Joost Nelis, Christopher T. Elliott |
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| Rok vydání: | 2020 |
| Předmět: |
02 engineering and technology
Biosensing Techniques 01 natural sciences Analytical Chemistry chemistry.chemical_compound Soot Okadaic Acid Animals SDG 14 - Life Below Water 14. Life underwater Electrodes Saxitoxin Immunoassay Chromatography Kainic Acid 010401 analytical chemistry Domoic acid Okadaic acid Electrochemical Techniques 021001 nanoscience & nanotechnology 0104 chemical sciences Dielectric spectroscopy chemistry Marine Toxins Differential pulse voltammetry Cyclic voltammetry 0210 nano-technology Marine toxin Biosensor |
| Zdroj: | Neils, J L D, Migliorelli, D, Muhlebach, L, Generelli, S, Stewart, L, Elliott, C T & Campbell, K 2021, ' Highly sensitive electrochemical detection of the marine toxins okadaic acid and domoic acid with carbon black modified screen printed electrodes ', Talanta, vol. 228, 122215 . https://doi.org/10.1016/j.talanta.2021.122215 Talanta |
| ISSN: | 1873-3573 |
| DOI: | 10.1016/j.talanta.2021.122215 |
| Popis: | A novel electrochemical immunosensor for the detection of the important marine biotoxins domoic acid (DA) and okadaic acid (OA) was developed. The sensors used carbon black modified screen-printed electrodes (CB-SPE) obtained using a high-throughput method. The electrochemical performance and stability of CB modified SPEs and bare carbon SPEs (c-SPEs) were compared using cyclic voltammetry and electrochemical impedance spectroscopy. CB-SPEs showed improved long-term (at least six months) stability and electro-catalytic properties compared with c-SPEs. The CB-SPEs were bio-functionalized with DA or OA protein-conjugates and used to develop two indirect competitive immunosensors using differential pulse voltammetry (DPV). The DPV signals obtained for the OA and DA immunosensors fitted well to four-parameter dose-response curves (R2 > 0.98) and showed excellent LODs (LOD = 1.7 ng mL−1 for DA in buffer; LOD = 1.9 ng mL−1 for DA in mussel extract; LOD = 0.15 ng mL−1 for OA in buffer; LOD = 0.18 ng mL−1 for OA in mussel extract). No significant interference of the naturally co-occurring marine toxins saxitoxin, tetrodotoxin and OA was detected for the DA immunosensor. Similarly, for the OA immunosensor saxitoxin, tetrodotoxin and DA did not cross-react and very limited interference was observed for the dinophysis toxins DTX-1, DTX-2 and DTX-3 (OA congeners). Moreover, both immunosensors remained stable after at least 25 days of storage at 4 °C. This work demonstrates the potential of affordable, mass-produced nanomaterial-modified SPEs for marine toxin detection in shellfish. |
| Databáze: | OpenAIRE |
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