Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation

Autor: Andrielle Castilho-Fernandes, Fernando Lucas Primo, Tácila Gabriele Lopes, Aparecida Maria Fontes, Valéria F. Silva, Fernanda U. Ferreira, Antonio Claudio Tedesco, Nayara Rezende, Alfredo Ribeiro-Silva
Přispěvatelé: Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Popis: Made available in DSpace on 2019-10-06T16:48:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-03-01 Financiadora de Estudos e Projetos Background: Bone marrow mesenchymal stem cells (BM-MSCs) are undifferentiated cells that can proliferate and differentiate into specialized cells for tissue self-repair. Low-level laser (LLL) can induce biomodulatory effects such as cellular proliferation, differentiation, and migration. We investigated the biomodulatory effects of the photoactive compound chloroaluminum phthalocyanine nanoemulsion (AlClPc/NE) on the adipogenic differentiation of BM-MSCs, when combined with LLL (AlClPc/NE-LLL). Methods: The BM-MSCs used in this work were isolated from green fluorescent protein-positive (GFP + ) C57BL6 mice. Cells were first treated with AlClPc/NE, a well-designed photoactive nano-drug and were then subjected to in vitro expansion, morphological and immunophenotypic characterization, and cellular cytotoxicity analysis. Subsequently, BM-MSCs were induced to differentiate into adipocytes by photo-induced biomodulation with AlClPc/NE-LLL. Results: Our results showed that the isolated cell population was consistent with murine BM-MSCs. The cellular cytotoxicity analysis revealed that the optimal nanoemulsion dose to induce BM-MSC biomodulation was 5.0 μmol/L. Twenty-four hours following treatment with AlClPc/NE, BM-MSC were subjected to visible light irradiation of 20 mJ/cm 2 at 670 nm. Six days after photo-induced biomodulation, cells maintained high GFP expression level, and expressed detectable mRNA levels of adipogenic genes (lipoprotein lipase and PPARγ); formation of lipid vacuoles was observed, and the cells did not show any tumorigenic potential in vivo. Conclusions: Our results indicated that photo-induced biomodulation via visible light using AlClPc/NE and LLL can induce adipogenic differentiation of murine BM-MSCs. Therefore, cell therapy with BM-MSCs and photo-induced biomodulation may contribute to the development of new therapeutic strategies that are faster and more effective than traditional methods to trigger MSC differentiation. Department of Chemistry Center of Nanotechnology and Tissue Engineering – Photobiology and Photomedicine Research Group Faculty of Philosophy Sciences and Letters of Ribeirão Preto Ribeirão Preto University of São Paulo Center for Cell Therapy and Regional Blood Center National Institute of Science and Technology in Stem Cell and Cell Therapy Medical School University of São Paulo Department of Pathology Medical School University of Sao Paulo Departament of Bioprocess and Biotechnology São Paulo State University (UNESP) School of Pharmaceutical Sciences Departament of de Genetics Medical School University of São Paulo Departament of Bioprocess and Biotechnology São Paulo State University (UNESP) School of Pharmaceutical Sciences Financiadora de Estudos e Projetos: 01.10.0758.01
Databáze: OpenAIRE
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