Sequencing and population analysis of four novel HLA-DPA1 alleles
| Autor: | Rui Ping Dong, Akinori Kimura, Haruhito Harada, Xing Pei Xu, Kuldeep Bhatia, Takehiko Sasazuki |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
HLA-DP Antigens
Genes MHC Class II Immunoblotting Molecular Sequence Data Immunology Population Human leukocyte antigen HLA-DP alpha-Chains Biology Polymerase Chain Reaction White People Cell Line law.invention Asian People Gene Frequency law Sequence Homology Nucleic Acid Humans Immunology and Allergy Amino Acid Sequence Cloning Molecular Allele education Gene Polyacrylamide gel electrophoresis Alleles Polymerase chain reaction Genetics education.field_of_study Base Sequence Sequence Homology Amino Acid Single-strand conformation polymorphism General Medicine Molecular biology Electrophoresis Polyacrylamide Gel Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Human Immunology. 35:173-178 |
| ISSN: | 0198-8859 |
| DOI: | 10.1016/0198-8859(92)90102-s |
| Popis: | We determined the base sequences of the HLA-DPA1 gene from four B-lymphoblastoid cell lines (CB6B, LKT3, AMAI, and T7526) that showed distinct electrophoretic patterns of single-stranded polymerase chain reaction products of the HLA-DPA1 gene. The novel HLA-DPA1 alleles of CB6B, LKT3, AMAI, and T7526 were designated DPA1∗02021, DPA1∗02022, DPA1∗0301, and DPA1∗0401, respectively. Although there was only one base substitution between DPA1∗02021 and DPA1∗02022, the single-strand conformation polymorphism of these two alleles was clearly demonstrated by electrophoresis in a nondenaturing polyacrylamide gel containing 10% glycerol. In addition, we genotyped for the HLA-DPA1 gene of healthy unrelated Oriental individuals—i.e., 227 Japanese, 88 Papua New Guineans, and 41 Buyi-Chinese—to demonstrate the ethnic distribution of the HLA-DPA1 alleles. |
| Databáze: | OpenAIRE |
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