Long-term in vivo integrity and safety of 3D-bioprinted cartilaginous constructs
| Autor: | Lars Kölby, Anders Lindahl, Karin Säljö, Kajsa Markstedt, Peter Apelgren, Paul Gatenholm, Linnéa Stridh Orrhult, Camilla Brantsing, Matteo Amoroso |
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| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Materials science 0206 medical engineering Biomedical Engineering Mice Nude 02 engineering and technology In Vitro Techniques Biomaterials 03 medical and health sciences Chondrocytes Fibrosis In vivo Osteogenesis medicine Cartilaginous Tissue Cell Adhesion Animals Humans 030304 developmental biology Cell Proliferation 0303 health sciences Mice Inbred BALB C Tissue Engineering Tissue Scaffolds Ossification Cartilage Stem Cells Cell Differentiation medicine.disease 020601 biomedical engineering Cell biology Biomechanical Phenomena Biological safety medicine.anatomical_structure Printing Three-Dimensional Female medicine.symptom Stem cell |
| Zdroj: | Journal of biomedical materials research. Part B, Applied biomaterialsREFERENCES. 109(1) |
| ISSN: | 1552-4981 |
| Popis: | Long-term stability and biological safety are crucial for translation of 3D-bioprinting technology into clinical applications. Here, we addressed the long-term safety and stability issues associated with 3D-bioprinted constructs comprising a cellulose scaffold and human cells (chondrocytes and stem cells) over a period of 10 months in nude mice. Our findings showed that increasing unconfined compression strength over time significantly improved the mechanical stability of the cell-containing constructs relative to cell-free scaffolds. Additionally, the cell-free constructs exhibited a mean compressive stress and stiffness (compressive modulus) of 0.04 +/- 0.05 MPa and 0.14 +/- 0.18 MPa, respectively, whereas these values for the cell-containing constructs were 0.11 +/- 0.08 MPa (p= .019) and 0.53 +/- 0.59 MPa (p= .012), respectively. Moreover, histomorphologic analysis revealed that cartilage formed from the cell-containing constructs harbored an abundance of proliferating chondrocytes in clusters, and after 10 months, resembled native cartilage. Furthermore, extension of the experiment over the complete lifecycle of the animal model revealed no signs of ossification, fibrosis, necrosis, or implant-related tumor development in the 3D-bioprinted constructs. These findings confirm the in vivo biological safety and mechanical stability of 3D-bioprinted cartilaginous tissues and support their potential translation into clinical applications. |
| Databáze: | OpenAIRE |
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