Marker of lipid peroxidation related to diabetic nephropathy in Indonesian type 2 diabetes mellitus patients
| Autor: | Da Hong Wang, Keiki Ogino, D.A. Saputri, A. Manfaatun, Heri Setiawan, Noriko Sakano, Dharmeizar, O.F. Amanda, Retnosari Andrajati, Rani Sauriasari, R.U. Muris, Azizahwati |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty Endocrinology Diabetes and Metabolism Renal function Enzyme-Linked Immunosorbent Assay Urine Dinoprost Diabetic nephropathy chemistry.chemical_compound Endocrinology Internal medicine Diabetes mellitus Malondialdehyde Internal Medicine medicine TBARS Prevalence Humans Diabetic Nephropathies Aged business.industry Type 2 Diabetes Mellitus General Medicine Middle Aged medicine.disease chemistry Diabetes Mellitus Type 2 Indonesia Spectrophotometry Creatinine Microalbuminuria Female Lipid Peroxidation business Biomarkers Glomerular Filtration Rate |
| Zdroj: | Diabetes research and clinical practice. 108(1) |
| ISSN: | 1872-8227 |
| Popis: | Objective Even though diabetes patients exhibit an increased oxidative stress, its correlation with diabetic nephropathy is not fully understood. The purpose of this study was to determine whether lipid peroxidation marker correlates well with eGFR and UACR in type 2 diabetes mellitus patients. Methods We collected urine and serum samples of Indonesian type 2 diabetes mellitus outpatients with normo- and microalbuminuria at a Local Government Clinic (from ages: 39–74 years). Urinary 8-iso-PGF 2α was measured by ELISA, the serum malondialdehyde by TBARS assay, and urinary albumin by BCG albumin assay. eGFR was calculated using the corrected-Cockcroft–Gault (CG), MDRD, and CKD-EPI equation. Other necessary data were obtained through questionnaires. Results The results showed that the increasing level of malondialdehyde was mildly correlated with the decline in eGFR (MDRD). In contrary, there was a significant positive correlation between 8-iso-PGF 2α concentration and eGFR based on the corrected-CG, MDRD study, and CKD-EPI equation ( r = 0.457, p r = 0.424, p r = 0.443, p n = 43) ( r = 0.491, p = 0.001; r = 0.461, p = 0.002; r = 0.455, p = 0.002). The multivariate analysis showed that 8-iso-PGF 2α together with fasting plasma glucose was the most predictive factor for the high 2-quantile eGFR (adjusted OR 1.001, (95% CI, 1.000–1.001)). However, there was no significant correlation between UACR with malondialdehyde ( r = 0.268, p = 0.050) and 8-iso-PGF 2α ( r = −0.030, p = 0.808). UACR itself was inversely correlated with eGFR based on the corrected-CG, the MDRD, and CKD-EPI ( r = −0.232, p r = −0.228, p r = −0.232, p Conclusions Increased 8-iso-PGF 2α and malondialdehyde in type 2 diabetes mellitus patients may play a role in the pathophysiologic significance of diabetic nephropathy, even while considering the effect of potential confounders. |
| Databáze: | OpenAIRE |
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