A novel dietary multi-strain yeast fraction modulates intestinal toll-like-receptor signalling and mucosal responses of rainbow trout (Oncorhynchus mykiss)

Autor:	Mathieu Castex, Eric Leclercq, Mark D. Rawling, Andrew D. Foey, Daniel L. Merrifield
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	0301 basic medicine Every Two Weeks Trout Physiology Gene Expression Yeast and Fungal Models Immune Receptors Biochemistry Immune Physiology Gene expression Medicine and Health Sciences Intestinal Mucosa Receptor Toll-like Receptors Innate Immune System Immune System Proteins Multidisciplinary biology Eukaryota 04 agricultural and veterinary sciences Interleukin 10 Experimental Organism Systems Osteichthyes Oncorhynchus mykiss Vertebrates Saccharomyces Cerevisiae Cytokines Medicine Anatomy Interleukin 1 receptor type I Research Article Signal Transduction medicine.medical_specialty Science Immunology Research and Analysis Methods Saccharomyces 03 medical and health sciences Model Organisms Internal medicine Genetics medicine Animals Immunity Mucosal Nutrition Organisms Fungi Biology and Life Sciences Proteins Cell Biology Molecular Development biology.organism_classification Yeast Diet Gastrointestinal Tract TLR2 Fish 030104 developmental biology Endocrinology Immune System Animal Studies 040102 fisheries 0401 agriculture forestry and fisheries Rainbow trout Digestive System Zoology Developmental Biology
Zdroj:	PLoS ONE, Vol 16, Iss 1, p e0245021 (2021) PLoS ONE
ISSN:	1932-6203
Popis:	This study was conducted to evaluate the mucosal immune responses of rainbow trout when supplementing an experimental formulated feed with multi-strain yeast fraction product (Saccharomyces cerevisiae and Cyberlindnera jardinii). In total, 360 fish (initial BW 23.1 ± 0.2 g) were randomly allotted into three dietary treatments in an 8-week feeding trial. The dietary treatments included basal diet (control) and control + 1.5 g/kg multi-strain yeast fraction product (MsYF) fed continuously and pulsed every two weeks between control and MsYF diet. No negative effects on growth performance of feeding the MsYF supplemented diet were observed. SGR and FCR averaged 2.30 ± 0.03%/day and 1.03 ± 0.03, respectively, across experimental groups. Muscularis thickness in the anterior intestine after 8 weeks of feeding was significantly elevated by 44.3% in fish fed the MsYF continuously, and by 14.4% in fish fed the MsYF pulsed (P < 0.02). Significant elevations in goblet cell density in the anterior and posterior (>50% increase) intestine were observed after 8 weeks of feeding the MsYF supplemented diet (P< 0.03). In contrast, lamina propria width was significantly lower in fish fed the experimental diets (>10% reduction). The gene expression analysis of the intestine revealed significant elevations in expression of tlr2, il1r1, irak4, and tollip2 after 4 weeks of feeding the MsYF. Significant elevations in effector cytokines tnfα, il10 and tgfβ were observed after 4 weeks of feeding the MsYF regime. After 8 weeks significant elevations in the gene expression levels of il1β, ifnγ, and il12 were observed in fish fed the MsYF. Likewise, the expression of the transcription factor gata3 was significantly elevated (P
Databáze:	OpenAIRE
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