RADIOIMMUNOASSAY FOR HUMAN GROWTH HORMONE USING MONOCLONAL ANTIBODIES
| Autor: | P. G. Bundesen, H. G. Friesen, A. H. Sehon, I. G. Worsley, K.A. Kelly, R. G. Drake |
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| Rok vydání: | 1980 |
| Předmět: |
Male
endocrine system medicine.medical_specialty medicine.drug_class Endocrinology Diabetes and Metabolism Clinical Biochemistry Radioimmunoassay Monoclonal antibody Biochemistry Antibodies Mice Endocrinology Human placental lactogen Internal medicine medicine Animals Ascitic Fluid Humans Placental lactogen biology Chemistry Human growth hormone Biochemistry (medical) Placental Lactogen Molecular biology Clone Cells Rats Polyclonal antibodies Growth Hormone embryonic structures biology.protein Cattle Female Antibody hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 51:1472-1474 |
| ISSN: | 1945-7197 0021-972X |
| Popis: | Radioimmunoassay (RIA's) for human growth hormone (hGH) employing monoclonal antibodies (Mab) were compared with a conventional clinical RIA using polyclonal antibodies (PAb). Several MAb displayed sensitivities (defined as the concentration of hormone required for 50% inhibition of binding of 125I-hGH) for hGH in the RIA which were equivalent to those of PAb. Those MAb with the greatest sensitivity for hGH displayed an equal affinity for human placental lactogen (hPL), whereas those with lower sensitivity for hGH, cross-reacted minimally with hPL. The concentrations of hGH in human serum samples were determined with MAb and the values showed a high degree of correlation with those obtained by PAb. It is therefore concluded that MAb are useful for the development of clinical RIA's for polypeptide hormones. |
| Databáze: | OpenAIRE |
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