Gene expression profiling of progenitor cells isolated from rat rotator cuff musculotendinous junction

Autor: Anna Plaas, Jun Li, Brian J. Cole, Mandeep S. Virk, Katie J. Sikes, Wei Luo
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Pathology
medicine.medical_specialty
Bone marrow cells
lcsh:Diseases of the musculoskeletal system
Primary Cell Culture
Adipose tissue
Cell Separation
Rotator Cuff Injuries
03 medical and health sciences
Rotator Cuff
0302 clinical medicine
Rheumatology
medicine
Animals
Humans
Orthopedics and Sports Medicine
Progenitor cell
Cells
Cultured
Progenitor
030222 orthopedics
Achilles tendon
Rotator cuff muscle
Wound Healing
business.industry
MSC gene expression profile
Gene Expression Profiling
Stem Cells
Mesenchymal stem cell
Rotator cuff tendon
030229 sport sciences
musculoskeletal system
Rotator cuff musculotendinous junction
Tendon
Rats
medicine.anatomical_structure
Feasibility Studies
Bone marrow
Stem cell
lcsh:RC925-935
business
Chondrogenesis
Biomarkers
Research Article
Stem Cell Transplantation
Zdroj: BMC Musculoskeletal Disorders
BMC Musculoskeletal Disorders, Vol 21, Iss 1, Pp 1-12 (2020)
ISSN: 1471-2474
Popis: Background Rotator cuff tendon tears are typically degenerative and usually affect the region of tendon insertion on bone. The remnant torn tendon is degenerative and may not be an ideal source for progenitor cells for cell-based therapies. Therefore, the aim of this study was to determine if musculotendinous junction (MTJ), which is adjacent to tendon would be a viable alternate source of progenitor stem cells. We also sought to study the gene expression profile MTJ progenitors and compare it with progenitors isolated from RC tendon, RC muscle and other existing tissue sources (bone marrow, adipose tissue, and Achilles tendon). Methods Rotator cuff tendon (RCT), muscle (RCM), and RCMTJ as well as Achilles tendon (AT) tissues were harvested from healthy male Lewis rats and progenitor cultures were established from these tissues and also from bone marrow and adipose tissue. Quantitative RT-PCR was performed on RNA extracts from intact tissues and progenitor cells using a custom array for the mesenchymal stem cell (MSC) differentiation marker genes. The gene expression profile of MSC differentiation markers within four tissues types, six progenitor cells, and between tissue and their corresponding progenitors were compared. Results Progenitors cells can be isolated from rat rotator cuff musculotendinous tissue and their pattern of MSC gene expression was similar to the rotator cuff tendon progenitors for majority of the genes tested. However, there were significant differences between the MSC gene expression patterns of RCMTJ and RCM progenitors. Furthermore, there were differences in gene expression between the RCMTJ tissue and its progenitor cells with respect to MSC differentiation markers. The gene expression pattern of RCMTJ tissue was similar to RCM tissue with respect to markers of chondrogenesis, myogenesis, tenogenesis, and MSC specific markers. Conclusion We demonstrate that the musculotendinous junction contains distinct set of progenitor cells and their MSC gene expression pattern is similar to rotator cuff tendon progenitors. RCMTJ progenitors will be an attractive option for cell-based regenerative treatment of chronic rotator cuff tears.
Databáze: OpenAIRE
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