Clinical significance of CCNE2 protein and mRNA expression in thyroid cancer tissues
| Autor: | Gang Chen, Yun He, Cui-Zhen Liu, Jin-Bo Peng, Hong Yang, Yu-Yan Pang, Wei Ma, Xiao-Fan Liu, Wan-Ping Guo, Peng Lin, Xiao-Li Huang |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Microarray Apoptosis medicine.disease_cause Cyclin-dependent kinase Cyclins Adenocarcinoma Follicular Biomarkers Tumor Tumor Cells Cultured Medicine Humans RNA Messenger Thyroid Neoplasms KEGG Cell Proliferation biology business.industry Kinase Cyclin-dependent kinase 2 General Medicine Cell cycle Middle Aged Prognosis Carcinoma Papillary Gene Expression Regulation Neoplastic Survival Rate Cyclin E2 biology.protein Cancer research Female business Carcinogenesis |
| Zdroj: | Advances in medical sciences. 65(2) |
| ISSN: | 1898-4002 |
| Popis: | Thyroid carcinoma (TC) is the most common endocrinal malignancy worldwide. Cyclin E2 (CCNE2), a member of the cyclin family, acts as a regulatory subunit of cyclin-dependent kinases (CDKs). It controls the transition of quiescent cells into the cell cycle, regulates the G1/S transition, promotes DNA replication, and activates CDK2. This study explored the role and potential molecular mechanisms of CCNE2 expression in TC tissues.Immunohistochemistry was used to evaluate the CCNE2 protein expression levels in TC. High-throughput data on CCNE2 in TC were obtained from RNA sequencing (RNA-seq), microarray, and literature data. The CCNE2 expression levels in TC were comprehensively assessed through an integrated analysis. Analyses of Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPIs) data facilitated the investigation of the relative molecular mechanisms of CCNE2 in TC.The immunohistochemical experiment showed a significant increase in the expression of CCNE2 in the TC tissues. For 505 TC and 59 non-cancerous samples from RNA-seq data, the area under the curve (AUC) was 0.8016 (95% confidence interval [CI] 0.742-0.8612; p0.001). With another 14 microarrays, the pool standard mean difference [SMD] was 1.01 (95% CI [0.82-1.19]). The pooled SMD of CCNE2 was 1.12 (95% CI [0.60-1.64]), and the AUC was 0.87 (95% CI [0.84-0.90]) for 1157 TC samples and 366 non-cancerous thyroid samples from all possible sources. Nine hub genes were upregulated in TC.A high expression of CCNE2 may lead to carcinogenesis and the development of TC. |
| Databáze: | OpenAIRE |
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