Cloning and functional analysis of autophagy-related gene 7 in Bombyx mori, silkworm
| Autor: | Piao Feng, Dandan Bian, Fanchi Li, Bing Li, Minli Dai, Qingyu Zhu, Yuying Ren, Tingting Mao, Wentao Ye, Zhiya Gu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology media_common.quotation_subject ATG8 01 natural sciences Biochemistry Autophagy-Related Protein 7 ATG12 03 medical and health sciences Bombyx mori RNA interference Animals ortho-Aminobenzoates Amino Acid Sequence Metamorphosis Cloning Molecular Gene media_common Cloning biology fungi General Medicine biology.organism_classification Bombyx Cell biology 010602 entomology Open reading frame 030104 developmental biology Ecdysterone Insect Science |
| Zdroj: | Archives of insect biochemistry and physiologyREFERENCES. 107(4) |
| ISSN: | 1520-6327 |
| Popis: | Silkworm (Bombyx mori) is an important economic insect and an attractive model system. A series of autophagy-related genes (Atgs) are involved in the autophagic process, and these Atgs have been proved to play important roles in the development. Atg7 stands at the hub of two ubiquitin-like systems involving Atg8 and Atg12 in the autophagic vesicle. In the present study, we cloned and characterized a BmAtg7 gene in Bombyx mori. The open reading frame (ORF) of BmAtg7 was 1908 bp in length, and it encoded a polypeptide of 635 amino acids. BmAtg7 was highly expressed in the posterior silk gland, fatbody, and epidermis. The expression profile of BmAtg7 in the fatbody showed an increasing tendency from day 1 of the 5th instar to the prepupal stage. After chlorantraniliprole (CAP) exposure, the transcriptional level of BmAtg7 was continuously decreased. After depletion of BmAtg7 by RNAi, the expressions of BmAtg7, BmAtg8, and BmEcr were all downregulated, while the expression of BmJHBP2 was upregulated. However, depletion of BmAtg7 did not prevent the metamorphosis of silkworm from larvae to pupae, while the occurrence of such process was delayed. After the 20-hydroxyecdysone (20E) treatment, the expression characteristics of these four genes (BmAtg7, BmAtg8, BmEcr and BmJHBP2) were contrary to the results after depletion of BmAtg7. Our results suggested that although CAP exposure could significantly inhibit the expression of BmAtg7 continuously, the changes of BmAtg7 was not the key factor in CAP-induced metamorphosis defects. |
| Databáze: | OpenAIRE |
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