Higher Effectiveness of New Common Bean (Phaseolus vulgaris L.) Germplasm Acquisition by Collecting Expeditions Associated with Molecular Analyses
| Autor: | Daniel Mihálik, Ján Kraic, Michal Šajgalík, Pavol Hauptvogel, Katarína Ondreičková, Miroslav Glasa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Germplasm Geography Planning and Development lcsh:TJ807-830 lcsh:Renewable energy sources Management Monitoring Policy and Law Biology 01 natural sciences Crop 03 medical and health sciences Genotype Genetic variability Cultivar collecting expedition Genotyping lcsh:Environmental sciences 030304 developmental biology common bean lcsh:GE1-350 0303 health sciences Genetic diversity Renewable Energy Sustainability and the Environment business.industry lcsh:Environmental effects of industries and plants food and beverages Building and Construction germplasm Biotechnology lcsh:TD194-195 Microsatellite private allele business 010606 plant biology & botany |
| Zdroj: | Sustainability, Vol 11, Iss 19, p 5270 (2019) Sustainability Volume 11 Issue 19 |
| ISSN: | 2071-1050 |
| Popis: | A remarkable portion of crop genetic diversity is represented by landraces and obsolete cultivars, that have not yet been integrated into the collections of genetic resources in genebanks. Fortunately, they are still maintained by small farmers in rural areas. Their acquisition is an important goal for collecting expeditions, followed by the evaluation of their genetic background. Molecular markers are powerful tools to evaluate the effectiveness of acquisition of new genetic variants. This approach was used for common bean landraces, that were collected through expeditions in the Slovak and Ukrainian Carpathians. In this study, microsatellite markers, developed from expressed sequence tags, were used for genotyping the collected materials. The sub-population of collected landraces contained higher total and average number of different alleles in comparison to equally large sub-populations of already-maintained cultivars. The same was true for the Shannon&rsquo s information index, observed heterozygosity, and expected heterozygosity. Both sub-populations showed the presence of private alleles: Average values of 0.500 and 0.833 private alleles per genotype were detected in landraces, and cultivars, respectively. Obtained results emphasized the importance of collecting expeditions to specific regions where landraces are cultivated, even at the present time. The effectiveness of the acquisition of new genetic variability can be determined by molecular tools, as confirmed by microsatellite markers in this study. |
| Databáze: | OpenAIRE |
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