A new mitochondrial fluorescent zinc sensor
| Autor: | Dien Ton-That, Kyle R. Gee, Stefano L. Sensi, John H. Weiss, Anca Rothe |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
inorganic chemicals
Physiology chemistry.chemical_element Zinc Biosensing Techniques Mitochondrion Biology Sensitivity and Specificity Mice Prosencephalon Microscopy Animals Molecular Biology Cells Cultured Fluorescent Dyes Brain Chemistry Microscopy Confocal Cationic polymerization Cell Biology Fluorescence Mitochondria enzymes and coenzymes (carbohydrates) chemistry Biochemistry Microscopy Fluorescence Xanthenes Cell culture biological sciences health occupations Biophysics bacteria Intracellular Visible spectrum |
| Zdroj: | Cell calcium. 34(3) |
| ISSN: | 0143-4160 |
| Popis: | A novel cationic fluorescent zinc (Zn2+) indicator (RhodZin-3) with nanomolar affinity for Zn2+ has been synthesized. RhodZin-3 exhibits large pH-independent fluorescence increases in the orange region of the visible wavelength spectrum with increasing zinc concentrations, and no sensitivity to physiologically relevant Ca2+ concentrations. Experiments in neuronal cell cultures show that RhodZin-3 effectively localizes into mitochondria and detects changes of intramitochondrial free Zn2+ ([Zn2+]m). |
| Databáze: | OpenAIRE |
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