Identification, characterization and purification of the lantibiotic staphylococcin T, a natural gallidermin variant
| Autor: | B, Furmanek, T, Kaczorowski, R, Bugalski, K, Bielawski, J, Bohdanowicz, A J, Podhajska, J, Bogdanowicz |
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| Rok vydání: | 1999 |
| Předmět: |
Staphylococcus
Molecular Sequence Data Peptide Applied Microbiology and Biotechnology Mass Spectrometry Heating Cell membrane Staphylococcus cohnii Bacteriocins medicine Nucleoid Amino Acid Sequence Chromatography High Pressure Liquid Antibacterial agent chemistry.chemical_classification Chromatography Base Sequence biology Fast protein liquid chromatography General Medicine Hydrogen-Ion Concentration Lantibiotics biology.organism_classification Anti-Bacterial Agents Micrococcus luteus medicine.anatomical_structure chemistry Biochemistry Isoelectric Focusing Peptides Bacteria Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 87:856-866 |
| ISSN: | 1365-2672 1364-5072 |
| DOI: | 10.1046/j.1365-2672.1999.00937.x |
| Popis: | Staphylococcin T (StT), an antibacterial agent produced by a Staphylococcus cohnii T strain, was purified to homogeneity by ammonium sulphate precipitation, gel filtration, cation exchange and fast performance liquid chromatography (FPLC). The final yield was about 20%, and over a 1000-fold increase in the specific activity was obtained. Mass determination (2166 Da), amino acid sequencing (Ile-Ala-Xaa-Lys-Phe-Leu-Xaa-Xaa-Pro-Gly-Xaa-Ala-Lys-block) and DNA sequencing demonstrated that StT is identical to gallidermin, a lanthionine-containing antimicrobial peptide. StT has a broad spectrum of bactericidal activity against Gram-positive and some Gram-negative bacteria. StT appears to damage cell membrane, and as a result causes an efflux of ions and an immediate block in macromolecular synthesis. Moreover, electron microscopic observations reveal morphological changes, with a loss of ribosomes and condensation of the nucleoid DNA. These changes are followed by a dissolution of the cell contents resulting in a bacterial ghost composed of seemingly intact cell walls with remnants of the cytoplasmic membrane and internal structure. Since StT exhibits antimicrobial activity especially against the Staphylococcus species, this compound may be of use in the treatment of staphylococcal infections. |
| Databáze: | OpenAIRE |
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