Shikonin protects against D-Galactosamine and lipopolysaccharide-induced acute hepatic injury by inhibiting TLR4 signaling pathway
| Autor: | Tong Zhang, Shanshan Huang, Lingxiang Jin, Guangyao Zhou, Yong-Xiang Yi, Chenwei Pan, Peipei Fang, Meng-Xiang Lin |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
LPS Lipopolysaccharide Pharmacology Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot Medicine TLR4 hepatic injury Liver injury shikonin biology medicine.diagnostic_test Traditional medicine business.industry Lithospermum erythrorhizon biology.organism_classification medicine.disease Naphthoquinone In vitro 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis business Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.21070 |
| Popis: | Shikonin, a naphthoquinone isolated from the root of medical herb Lithospermum erythrorhizon, has been reported to have anti-inflammatory effect. However, there is no related research for the treatment of shikonin on hepaic injury. The purpose of this study was to investigate the effects of shikonin on D-Galactosamine and Lipopolysaccharide-induced hepatic injury in mice. Male BALB/c mice were pretreated with shikonin 1 h before LPS/D-GalN treatment. The pathological changes of hepatic injury were detected by H&E staining. The levels of TNF-α and IL-1β in hepatic tissues were detected by ELISA. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were also measured in this study. In addition, the expression of TLR4 and NF-κB were determined by western blot analysis. These results suggest that shikonin effectively prevents LPS/D-GalN-induced liver injury by inhibiting AST and ALT levels, as well as inflammatory cytokines TNF-α and IL-1β production. The expression of TLR4 and NF-κB activation induced by LPS/D-GalN were also inhibited by treatment of shikonin. In vitro, shikonin significantly inhibited LPS-induced TNF-α and IL-1β production, as well as TLR4 expression and NF-κB activation. In conclusion, the results of the present study suggest that shikonin attenuates LPS/D-GalN-induced hepatic injury by inhibiting TLR4 signaling pathway. |
| Databáze: | OpenAIRE |
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