Synergistic Interactions of Sugars/Polyols and Monovalent Salts with Phospholipids Depend upon Sugar/Polyol Complexity and Anion Identity
Autor: | Jaroslaw Majewski, Bulent Akgun, Charles T.R. Heffern, J. Michael Henderson, Ginevra A. Clark, Ka Yee C. Lee |
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Rok vydání: | 2015 |
Předmět: |
Glycerol
Cation binding Langmuir 1 2-Dipalmitoylphosphatidylcholine Sodium Inorganic chemistry chemistry.chemical_element Sodium Chloride chemistry.chemical_compound Polyol Electrochemistry General Materials Science Sugar Spectroscopy chemistry.chemical_classification technology industry and agriculture Trehalose Water Stereoisomerism Surfaces and Interfaces Condensed Matter Physics Glucose chemistry Dipalmitoylphosphatidylcholine lipids (amino acids peptides and proteins) |
Zdroj: | Langmuir : the ACS journal of surfaces and colloids. 31(46) |
ISSN: | 1520-5827 |
Popis: | We found that interactions of dipalmitoylphosphatidylcholine (DPPC) lipid monolayers with sugars are influenced by addition of NaCl. This work is of general importance in understanding how sugar-lipid-salt interactions impact biological systems. Using Langmuir isothermal compressions, fluorescence microscopy, atomic force microscopy, and neutron reflectometry, we examined DPPC monolayers upon addition of sugars/polyols and/or monovalent salts. Sugar-lipid interactions in the presence of NaCl increased with increasing complexity of the sugar/polyol in the order glycerol ≪ glucosetrehalose. When the anion was altered in the series NaF, NaCl, and NaBr, only minor differences were observed. When comparing LiCl, NaCl, and KCl, sodium chloride had the greatest influence on glucose and trehalose interactions with DPPC. We propose that heterogeneity created by cation binding allows for sugars to bind the lipid headgroups. While cation binding increases in the order K(+)Na(+)Li(+), lithium ions may also compete with glucose for binding sites. Thus, both cooperative and competitive factors contribute to the overall influence of salts on sugar-lipid interactions. |
Databáze: | OpenAIRE |
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