Ebola Virus Aptamers: From Highly Efficient Selection to Application on Magnetism-Controlled Chips
| Autor: | Dai-Wen Pang, Zhi-Ling Zhang, Meng-Qi Xiang, Man Tang, Shao-Li Hong |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Ebola virus
Chemistry Aptamer Point-of-Care Systems 010401 analytical chemistry SELEX Aptamer Technique Computational biology Aptamers Nucleotide Hemorrhagic Fever Ebola 010402 general chemistry medicine.disease_cause Ebolavirus 01 natural sciences Signal acquisition 0104 chemical sciences Analytical Chemistry Magnetics Viral Proteins Limit of Detection medicine Humans Selection (genetic algorithm) |
| Zdroj: | Analytical chemistry. 91(5) |
| ISSN: | 1520-6882 |
| Popis: | Aptamers for Ebola virus (EBOV) offer a powerful means for prevention and diagnostics. Unfortunately, few aptamers for EBOV have been discovered yet. Herein, assisted by magnetism-controlled selection chips to strictly manipulate selection conditions, a highly efficient aptamer selection platform for EBOV is proposed. With highly stringent selection conditions of rigorous washing, manipulation of minuscule amounts of magnetic beads, and real-time evaluation of the selection effectiveness, the selection performance of the platform was improved significantly. In only three rounds of selection, the high-performance aptamers for EBOV GP and NP proteins were obtained simultaneously, with dissociation constants ( Kd) in the nanomolar range. The aptamer was further applied to the detection of EBOV successfully, with a detection limit of 4.2 ng/mL. The whole detection process that consisted of sample mixing, separation, and signal acquisition was highly integrated and conducted in a magnetism-controlled detection chip, showing high biosafety and great potential for point-of-care detection. The method may open up new avenues for prevention and control of EBOV. |
| Databáze: | OpenAIRE |
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