Frequency of first and second-line drug resistance-associated mutations among resistant Mycobacterium tuberculosis clinical isolates from São Paulo, Brazil
| Autor: | Sylvia Cardoso Leão, Tania Matsui, Angela Pires Brandão, Juliana Maira Watanabe Pinhata, Michelle Christiane da Silva Rabello, Cristina Viana-Niero, Rosangela Siqueira de Oliveira, Lucilaine Ferrazoli |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Microbiology (medical) Tuberculosis Adolescent Extensively Drug-Resistant Tuberculosis 030231 tropical medicine RC955-962 Antitubercular Agents gyrA MDR-TB gyrB Microbial Sensitivity Tests Drug resistance medicine.disease_cause Microbiology Bacterial genetics Mycobacterium tuberculosis Young Adult 03 medical and health sciences 0302 clinical medicine Drug Resistance Multiple Bacterial Arctic medicine. Tropical medicine Tuberculosis Multidrug-Resistant Humans Medicine XDR-TB pre-XDR-TB Gene Mutation biology business.industry Middle Aged biology.organism_classification rpoB medicine.disease Virology QR1-502 rrs Socioeconomic Factors Infectious disease (medical specialty) Original Article Female business Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 115 (2020) Memórias do Instituto Oswaldo Cruz |
| ISSN: | 1678-8060 |
| Popis: | BACKGROUND Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis, and the number of new cases of multidrug resistant TB (MDR-TB), pre extensively drug-resistant TB (pre-XDR-TB) and extensively drug-resistant TB (XDR-TB) has increased considerably worldwide. OBJECTIVES Herein, using 156 M. tuberculosis isolates from 106 patients previously classified as MDR or pre-XDR or XDR isolates, we investigated the genetic mutation profiles associated with phenotypic resistances in patients with MDR-TB, pre-XDR-TB and XDR-TB, treatment outcomes and resistance evolution. METHODS Molecular analyses were performed by partial sequencing of the rpoB, katG, gyrA, gyrB, rrs genes and analysis of the fabG-inhA promoter region. Clinical, epidemiologic and demographic data were obtained from the TB Notification database system of São Paulo (TB-WEB) and the Information System for Special Tuberculosis Treatments (SITE-TB). FINDINGS Drug resistance was attributed to previously known mutations and a novel Asp449Val mutation in gyrB was observed in four isolates from the same patient. Ten patients had more than one isolate evaluated and eight of these patients displayed resistance progression. MAIN CONCLUSIONS The present study is the first to report the frequency of mutations related to second-line drug resistance in MDR-TB, pre-XDR-TB and XDR-TB isolates. The results could lead to the improvement of available technologies for the rapid detection of drug resistant TB. |
| Databáze: | OpenAIRE |
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