Functional and Structural Assessment of the Effect of Human Umbilical Cord Blood Mesenchymal Stem Cells in Doxorubicin‐Induced Cardiotoxicity
| Autor: | Samia Hussein, Laila M. E. Sabik, Mai M. Hasan, Somia H. Abd Allah, Raghda H. A. Deraz, Wafaa F. Hussein |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Cardiomyopathy macromolecular substances Pharmacology Mesenchymal Stem Cell Transplantation Biochemistry Umbilical cord Umbilical Cord 03 medical and health sciences chemistry.chemical_compound Fibrosis Troponin I medicine Animals Humans Doxorubicin Molecular Biology Cardiotoxicity business.industry Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology medicine.disease Malondialdehyde Rats 030104 developmental biology medicine.anatomical_structure chemistry Immunology Heterografts Cord Blood Stem Cell Transplantation Cardiomyopathies business medicine.drug |
| Zdroj: | Journal of Cellular Biochemistry. 118:3119-3129 |
| ISSN: | 1097-4644 0730-2312 3119-3129 |
| DOI: | 10.1002/jcb.26168 |
| Popis: | Cardiomyopathy induced by doxorubicin (DOX) was recognized at an early stage and also several years after drug administration. Mesenchymal stem cells (MSCs) have many properties that make them suitable for preventive and/or regenerative therapies. In this study, we evaluated the effect of MSCs in the functional and the structural improvement of DOX-induced cardiomyopathy in rats. Ninety adult male albino rats were randomly divided into three equal groups of thirty rats each: Group I (control): rats received normal saline. Group II (DOX- group): rats received DOX. Group III (DOX-MSCs group): rats received DOX for 2 weeks then human umbilical cord blood mesenchymal stem cells (hUCB-MSCs). Rats in all groups were evaluated for: physical condition, electrocardiography (ECG), and hemodynamic parameters. Serum cardiac troponin I (cTnI), malondialdehyde (MDA), total antioxidant capacity (TAC), and DNA fragmentation on heart tissue isolated DNA were estimated for evaluation of the mechanism and the extent of the damage. Hearts were examined histopathologically for detection of MSCs homing, structural evaluation, with counting of the collagen fibers for evaluation of fibrosis. DOX-administered rats showed significant functional and structural deterioration. DOX-MSCs treated rats (group III) showed improved functional and structural criteria with restoration of all biochemical indicators of cardiac damage and reactive oxygen species (ROS) to normal, as well. In Conclusion, hUCB-MSCs significantly ameliorated the cardiotoxic manifestations as shown by biochemical, functional, and structural cardiac improvement. J. Cell. Biochem. 118: 3119-3129, 2017. © 2017 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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