Molecular subtyping of Neisseria meningitidis serogroup B: comparison of five methods
| Autor: | B. Swaminathan, Gloria W. Ajello, H Dronavalli, Wallis E. DeWitt, Michael W. Reeves, Susan B. Hunter, Leta O. Helsel, Ghassan M. Matar, Lewis M. Graves, W F Bibb, M el-Swify, M Morales |
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| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Serotype Neisseria meningitidis Biology medicine.disease_cause Polymerase Chain Reaction Disease Outbreaks Microbiology Ribotyping Pulsed-field gel electrophoresis medicine Humans Typing Serotyping Molecular Epidemiology Molecular epidemiology Antibodies Monoclonal bacterial infections and mycoses Virology Subtyping Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Enzymes Meningococcal Infections Evaluation Studies as Topic RRNA Operon Polymorphism Restriction Fragment Length Research Article |
| Zdroj: | Journal of Clinical Microbiology. 34:1468-1473 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | In order to compare methods for subtyping Neisseria meningitidis serogroup B isolates, 96 isolates obtained from various locations in the United States and northwestern Europe were subtyped by five methods: monoclonal antibody (MAb)-based serotyping and serosubtyping, DNA macrorestriction analysis by pulsed-field gel electrophoresis (PFGE), multilocus enzyme electrophoresis (MEE), ribotyping, and PCR-restriction fragment length polymorphism of the internally transcribed spacer region of the rRNA operon (ITS PCR-RFLP). All N. meningitidis serogroup B isolates were typeable by PFGE, MEE, ribotyping, and ITS PCR-RFLP. Only 44.8% of the isolates were completely typeable (both serotype and serosubtype determination) by MAb-based serotyping and serosubtyping. 60.4% of the isolates could be serotyped but not serosubtyped, and 90.6% of the isolates could be either serotyped or serosubtyped. Simpson's discrimination indices of diversity for the methods were as follows: PFGE, 99.7%; MEE, 99.4%; ribotyping, 98.8%; MAb serotyping, 75.8%; MAb serotyping and/or serosubtyping 97.5%; and ITS PCR-RFLP, 84.2%. The high degree of diversity observed by PFGE, MEE, and ribotyping can be explained by the fact that isolates were collected from different geographic locations at various times. PFGE, MEE, and ribotyping showed greater discriminatory abilities than MAb-based serotyping and serosubtyping or ITS PCR-RFLP. |
| Databáze: | OpenAIRE |
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