Colitogenic and non-colitogenic commensal bacteria differentially trigger DC maturation and Th cell polarization: an important role for IL-6
| Autor: | Manfred B. Lutz, Frauke Kahl, Ingo B. Autenrieth, Jörg Reimann, Carsten J. Kirschning, Martina Müller, Julia S. Frick, Erwin Bohn, Oliver Bechtold, Burghard Jilge, Naima Zahir |
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| Rok vydání: | 2006 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male T cell Immunology Priming (immunology) Stimulation Mice Transgenic Biology medicine.disease_cause Proinflammatory cytokine Microbiology Flow cytometry Immunophenotyping Mice fluids and secretions medicine Escherichia coli Immunology and Allergy Animals Bacteroides Secretion Colitis Escherichia coli Infections Mice Knockout Mice Inbred BALB C medicine.diagnostic_test Interleukin-6 Tumor Necrosis Factor-alpha Dendritic Cells medicine.disease Bacteroides Infections Flow Cytometry Interleukin-12 Cell biology Interleukin-10 Specific Pathogen-Free Organisms Mice Inbred C57BL medicine.anatomical_structure Female |
| Zdroj: | European journal of immunology. 36(6) |
| ISSN: | 0014-2980 |
| Popis: | We investigated whether commensal bacteria modulate activation and maturation of bone marrow-derived DC and their ability to prime CD4(+) T cells. We used Escherichia coli mpk, which induces colitis in gnotobiotic IL-2-deficient (IL-2(-/-)) mice, and Bacteroides vulgatus mpk, which prevents E. coli-induced colitis. Stimulation of DC with E. coli induced TNF-alpha, IL-12 and IL-6 secretion and expression of activation markers. Moreover, stimulation of DC with E. coli increased T cell activation and led to Th1 polarization. Stimulation with B. vulgatus led only to secretion of IL-6, and DC were driven into a semi-mature state with low expression of activation markers and did not promote Th1 responses. B. vulgatus-induced semi-mature DC were non-responsive to stimulation by E. coli in terms of maturation, T cell priming and TNF-alpha but not IL-6 production. The non-responsiveness of B. vulgatus-stimulated DC was abrogated by addition of anti-IL-6 mAb or mimicked with rIL-6. These data suggest that B. vulgatus-induced IL-6 drives DC into a semi-mature state in which they are non-responsive to proinflammatory activation by E. coli. This in vitro mechanism might contribute to the prevention of E. coli-triggered colitis development by B. vulgatus in vivo; high IL-6 mRNA expression was consistently found in B. vulgatus-colonized or B. vulgatus/E. coli co-colonized IL-2(-/-) mice and was associated with absence of colitis. |
| Databáze: | OpenAIRE |
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