Preparing protein microarrays by soft-landing of mass-selected ions
| Autor: | Justin M. Wiseman, Thomas A. Blake, Andy J. Guymon, Zheng Ouyang, Bogdan Gologan, Justin C. Oliver, V. Jo Davisson, R. Graham Cooks, Zoltan Takats |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray ionization Protein Array Analysis Oligosaccharides Cytochrome c Group Mass spectrometry Mass Spectrometry chemistry.chemical_compound Catalytic Domain Hexokinase medicine Insulin Trypsin Phosphorylation Neurotensin Ions Multidisciplinary Chromatography Myoglobin Proteins Cyclic AMP-Dependent Protein Kinases Matrix-assisted laser desorption/ionization chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Mass spectrum Protein microarray Muramidase Lysozyme Apoproteins Oligopeptides medicine.drug |
| Zdroj: | Science (New York, N.Y.). 301(5638) |
| ISSN: | 1095-9203 |
| Popis: | Intact, multiply protonated proteins of particular mass and charge were selected from ionized protein mixtures and gently landed at different positions on a surface to form a microarray. An array of cytochrome c, lysozyme, insulin, and apomyoglobin was generated, and the deposited proteins showed electrospray ionization mass spectra that matched those of the authentic compounds. Deposited lysozyme and trypsin retained their biological activity. Multiply charged ions of protein kinase A catalytic subunit and hexokinase were also soft-landed into glycerol-based liquid surfaces. These soft-landed kinases phosphorylated LRRASLG oligopeptide and D-fructose, respectively. |
| Databáze: | OpenAIRE |
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