Diversity analysis of gut microbiota between healthy controls and those with atopic dermatitis in a Chinese population
| Autor: | Yu Zhang, Xiumei Mo, Siqi Ye, Fenggen Yan, Junfeng Liu, Hongyi Li, Haiyan Wang, Dacan Chen |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Operational taxonomic unit China Adolescent Porphyromonadaceae Prevotella Physiology Dermatology Gut flora Dermatitis Atopic 030207 dermatology & venereal diseases 03 medical and health sciences Young Adult 0302 clinical medicine Clostridium RNA Ribosomal 16S medicine Bacteroides Humans Child Bacteroidaceae Feces biology business.industry General Medicine Atopic dermatitis biology.organism_classification medicine.disease Parabacteroides Gastrointestinal Microbiome 030220 oncology & carcinogenesis business |
| Zdroj: | The Journal of dermatologyReferences. 48(2) |
| ISSN: | 1346-8138 |
| Popis: | An increasing body of evidence suggests that gut microbiota is involved in atopic dermatitis (AD). We aimed to use high-throughput sequencing to characterize the differences in the composition of the gut microbiota between healthy controls and patients with AD. Fecal samples from 93 volunteers were analyzed using 16S rRNA sequencing, including 44 patients with AD and 49 healthy control subjects, aged 6-22 years. Our data show that the operational taxonomic unit composition in patients with AD had greater component similarity than the healthy controls. Patients with AD had a lower alpha diversity than healthy control subjects. The relative abundance of Porphyromonadaceae, Blautia, Parabacteroides, Bacteroides ovatus, Bacteroides uniformis and Prevotella stercorea was significantly higher (P < 0.05) in patients with AD than healthy control subjects. Clostridium and P. stercorea were higher (P < 0.05) in healthy control subjects compared with patients with AD. The results of linear discriminant analysis effect size show that Bacteroidaceae and Porphyromonadaceae can act as possible biomarkers associated with diagnosis of AD. However, this needs further experimental verification. Taken together, these results demonstrate the changes in microbiota composition in AD compared with a healthy control group, opening the way to future diagnosis or intervention studies. |
| Databáze: | OpenAIRE |
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