Citrus Flavanones Upregulate Thyrotroph Sirt1 and Differently Affect Thyroid Nrf2 Expressions in Old-Aged Wistar Rats
| Autor: | Marko Miler, Dragan Milenkovic, Ivana Jarić, Vladimir Ajdžanović, Jasmina Živanović, Branka Šošić-Jurjević, Verica Milošević |
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| Přispěvatelé: | Institute for Biological Research Sinisa Stankovic [Belgrade] (IBISS), University of Belgrade [Belgrade], Unité de Nutrition Humaine (UNH), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Bern, Ministry of Education, Science, and Technological Development of the Republic of Serbia 451-03-68/2020-14/200007 |
| Rok vydání: | 2020 |
| Předmět: |
Male
0106 biological sciences Naringenin Aging Citrus Antioxidant medicine.medical_treatment thyroid peroxidase Thyroid Gland 01 natural sciences chemistry.chemical_compound Sirtuin 1 antioxidant enzymes education.field_of_study Thyroid peroxidase biology hesperetin Thyroid Hesperetin food and beverages Thyroid ultrastructure medicine.anatomical_structure Flavanones Antioxidant enzymes General Agricultural and Biological Sciences Flavanone medicine.medical_specialty thyroid ultrastructure NF-E2-Related Factor 2 Population naringenin Internal medicine medicine Animals Rats Wistar education Plant Extracts 010401 analytical chemistry General Chemistry Rats 0104 chemical sciences Endocrinology chemistry Fruit biology.protein 570 Life sciences [CHIM.OTHE]Chemical Sciences/Other 010606 plant biology & botany Hormone |
| Zdroj: | Journal of Agricultural and Food Chemistry Journal of Agricultural and Food Chemistry, American Chemical Society, 2020, 68 (31), pp.8242-8254. ⟨10.1021/acs.jafc.0c03079⟩ Journal of Agricultural and Food Chemistry, 2020, 68 (31), pp.8242-8254. ⟨10.1021/acs.jafc.0c03079⟩ |
| ISSN: | 0021-8561 1520-5118 |
| Popis: | International audience; A growing population of elderly people consume citrus flavanones, naringenin, and hesperetin in the form of fruits or juices. Flavanones are bioactives with potent antioxidant properties and have potential in slowing down the aging process. Because flavanones exert controversial effects on pituitary-thyroid functioning, our study on the old-aged rat model aimed to elucidate the mechanism by which naringenin and hesperetin affect this axis. Naringenin and hesperetin increased the Sirt1 mRNA level by 91 and 71% (p < 0.05), which was followed by increased Sirt1 expression by 20 and 15% (p < 0.05), respectively. Only naringenin decreased thyroid-stimulating hormone expression by 20% (p < 0.05). Thyroid peroxidase protein expression was upregulated after naringenin or hesperetin by 62 and 43% (p < 0.05), respectively. Naringenin lowered mRNA levels of Tpo, Sod1, Sod2, Cat, and Nrf2 by 50, 32, 45, 35, and 42% (p < 0.05), respectively, and increased Gpx by 54% (p < 0.05), while hesperetin decreased Sod1 and Sod2 mRNA levels by 46 and 55% (p < 0.05), respectively. Naringenin increased the protein expressions of Nrf2 and SOD2 by 58 and 50% (p < 0.05), respectively, and decreased SOD1 expression by 48% (p < 0.05), while hesperetin protein decreased expressions of SOD1 and Nrf2 by 63 and 32% (p < 0.05), respectively. Altogether, our findings suggest that citrus flavanones contribute to restoring the impaired thyroid functioning in the old-aged rats. |
| Databáze: | OpenAIRE |
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