Quantification of CGRP‐immunoreactive myenteric neurons in mouse colon

Autor: Timothy J. Hibberd, Wai Ping Yew, Kelsi N. Dodds, Zili Xie, Lee Travis, Simon J. Brookes, Marcello Costa, Hongzhen Hu, Nick J. Spencer
Rok vydání: 2022
Předmět:
Zdroj: Journal of Comparative Neurology. 530:3209-3225
ISSN: 1096-9861
0021-9967
DOI: 10.1002/cne.25403
Popis: Quantitative data of biological systems provide valuable baseline information for understanding pathology, experimental perturbations, and computational modeling. In mouse colon, calcitonin gene-related peptide (CGRP) is expressed by myenteric neurons with multiaxonal (Dogiel type II) morphology, characteristic of intrinsic primary afferent neurons (IPANs). Analogous neurons in other species and gut regions represent 5-35% of myenteric neurons. We aimed to quantify proportions of CGRP-immunopositive (CGRP+) myenteric neurons. Colchicine-treated wholemount preparations of proximal, mid, and distal colon were labeled for HuC/D, CGRP, nitric oxide synthase (NOS), and peripherin (Per). The pan-neuronal markers (Hu+/Per+) co-labeled 94% of neurons. Hu+/Per- neurons comprised ∼6%, but Hu-/Per+ cells were rare. Thus, quantification was based on Hu+ myenteric neurons (8576 total; 1225 ± 239 per animal, n = 7). CGRP+ cell bodies were significantly larger than the average of all Hu+ neurons (329 ± 13 vs. 261 ± 12 μm
Databáze: OpenAIRE
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