Evaluating the relationship between acidogenicity and acid tolerance for oral streptococci from children with or without a history of caries
| Autor: | Jeffrey A. Banas, Min Zhu, Erika Takanami, Alissa L. Villhauer, Amber Marolf, David R. Drake, Fang Qian, Ryan M. Hemsley |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Oral streptococci education acid tolerance Physiology Infectious and parasitic diseases RC109-216 030206 dentistry Biology Microbiology QR1-502 stomatognathic diseases 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Infectious Diseases Acid tolerance acidogenicity dental caries Etiology Original Article Dentistry (miscellaneous) Microbiome Dominance (genetics) |
| Zdroj: | Journal of Oral Microbiology Journal of Oral Microbiology, Vol 12, Iss 1 (2020) |
| ISSN: | 2000-2297 |
| DOI: | 10.1080/20002297.2019.1688449 |
| Popis: | Background: Dental caries etiology is attributed to a dysbiotic imbalance within the plaque microbiome leading to a dominance of strong acidogens. Some studies that investigate the link between acidogens and caries quantify the recovery of acid tolerant strains on acid agar as a measure of acidogenic potential. This methodology assumes that acidogenic potential and acid tolerance are directly related. Aim: The validity of that assumption was investigated by statistically evaluating that relationship using streptococci recovered from children with or without a history of dental caries. Methods: Thirty streptococcal isolates were isolated from each of 13 subjects. Acidogenicity was quantified by measuring the terminal pH after overnight growth in Brain Heart Infusion (BHI) and Chemically Defined Medium (CDM). Acid tolerance was quantified by measuring the lowest pH acid agar displaying growth. Results: A significant difference in acidogenicity in CDM between levels of acid tolerance was found, but no significant difference in acidogenicity in BHI was noted. Moreover, there were no significant interactions between acid tolerance and caries history on acidogenicity measures in either medium. Conclusion: An ability to grow on acid agar below pH 5.0 is best aligned with strong acidogenicity and best able to distinguish between subjects with differing caries histories. |
| Databáze: | OpenAIRE |
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