Low-level inversion of the L component of pseudorabies virus is not dependent on sequence homology
| Autor: | Sabina Kupershmidt, T C Mettenleiter, X Q Lu, G F Rall, Tamar Ben-Porat |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
viruses
Molecular Sequence Data Restriction Mapping Immunology Mutant Genome Viral Transfection Cleavage (embryo) Microbiology Genome Virus Cell Line chemistry.chemical_compound Restriction map Sequence Homology Nucleic Acid Virology Alphaherpesvirinae Animals Chromosomal inversion Genetics Base Sequence biology Virion biology.organism_classification Herpesvirus 1 Suid Molecular biology chemistry Insect Science Chromosome Inversion DNA Viral DNA Research Article |
| Zdroj: | Journal of Virology. 65:7016-7019 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.65.12.7016-7019.1991 |
| Popis: | Pseudorabies virus has a class 2 genome in which the S component is found in two orientations relative to the L component. The L component is bracketed by sequences that are partially homologous; it is found mainly in one orientation, but a small proportion is inverted (J. M. DeMarchi, Z. Lu, G. Rall, S. Kuperschmidt, and T. Ben-Porat, J. Virol. 64:4968-4977, 1990). We have ascertained the role of the patchy homologous sequences bracketing the L component in its inversion. A viral mutant, vYa, from which the sequences at the right end of the L component were deleted was constructed. Despite the absence of homologous sequences bracketing the L component in vYa, its L component inverted to an extent similar to that of the L component in the wild-type virus. These results show the following. (i) The low-frequency inversion of the L component of PrV is not mediated by homologous sequences bracketing this component. (ii) Cleavage of concatemeric DNA at the internal junction between the S and L components is responsible for the appearance of the minority of genomes with an inverted L component in populations of pseudorabies virus. (iii) The signals present near or at the end of the S component are sufficient to allow low-frequency cleavage of concatemeric DNA; the sequences at the end of the L component are not essential for cleavage, although they enhance it considerably. |
| Databáze: | OpenAIRE |
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