Odd skipped-related 1 (Osr1) identifies muscle-interstitial fibro-adipogenic progenitors (FAPs) activated by acute injury
| Autor: | Sigmar Stricker, David Sassoon, Sophie vom Hofe-Schneider, Pedro Vallecillo Garcia, David Ollitrault, Jürgen Stumm, Aris N. Economides, Heinrich Schrewe, Giovanna Marazzi |
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| Přispěvatelé: | Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities [SDV]Life Sciences [q-bio] Induced Pluripotent Stem Cells Adipose tissue Skeletal muscle Endogeny Biology 03 medical and health sciences 0302 clinical medicine Muscle regeneration medicine Humans Progenitor cell Muscle Skeletal Transcription factor lcsh:QH301-705.5 neoplasms Cells Cultured 030304 developmental biology Muscle interstitium Gene Editing 0303 health sciences Cluster of differentiation Cysts Liver Diseases Mesenchymal stem cell Calcium-Binding Proteins Intracellular Signaling Peptides and Proteins Cell Differentiation Flow Cytometry digestive system diseases Cell biology medicine.anatomical_structure lcsh:Biology (General) 500 Naturwissenschaften und Mathematik::540 Chemie::547 Organische Chemie Gene Expression Regulation Hepatocyte Nuclear Factor 4 Adipogenesis Mesenchymal progenitors 500 Naturwissenschaften und Mathematik::570 Biowissenschaften Biologie::572 Biochemie CRISPR-Cas Systems Fibro-adipogenic progenitors 030217 neurology & neurosurgery Glucosidases Transcription Factors |
| Zdroj: | Stem Cell Research Stem Cell Research, Elsevier, 2018, 32, pp.8-16. ⟨10.1016/j.scr.2018.08.010⟩ Stem Cell Research, Vol 32, Iss, Pp 8-16 (2018) |
| ISSN: | 1876-7753 |
| DOI: | 10.1016/j.scr.2018.08.010⟩ |
| Popis: | Fibro-adipogenic progenitors (FAPs) are resident mesenchymal progenitors in adult skeletal muscle that support muscle repair, but also give rise to fibrous and adipose infiltration in response to disease and chronic injury. FAPs are currently identified using cell surface markers that do not distinguish between quiescent FAPs and FAPs actively engaged in the regenerative process. We have shown previously that FAPs are derived from cells that express the transcription factor Osr1 during development. Here we show that adult FAPs express Osr1 at low levels and frequency, however upon acute injury FAPs reactivate Osr1 expression in the injured tissue. Osr1+ FAPs are enriched in proliferating and apoptotic cells demonstrating that Osr1 identifies activated FAPs. In vivo genetic lineage tracing shows that Osr1+ activated FAPs return to the resident FAP pool after regeneration as well as contribute to adipocytes after glycerol-induced fatty degeneration. In conclusion, reporter LacZ or eGFP-CreERt2 expression from the endogenous Osr1 locus serves as marker for FACS isolation and tamoxifen-induced manipulation of activated FAPs.Summary statementExpression of Osr1 specifically in muscle interstitial fibro-adipogenic progenitors (FAPs) activated by acute injury provides a tool to isolate and trace this population. |
| Databáze: | OpenAIRE |
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