Transfer of small plasmid DNA fragments from polyacrylamide gels onto nitrocellulose paper
| Autor: | Kenneth L. Sisco, Donald L. Rucknagel, Philippe M. Frossard |
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| Rok vydání: | 1983 |
| Předmět: |
Paper
Plasmid preparation Chromatography Microchemistry Polyacrylamide Biophysics Collodion Nucleic Acid Hybridization DNA DNA Restriction Enzymes Cell Biology Biochemistry Molecular biology chemistry.chemical_compound chemistry Acrylamide Reagent Nucleic acid Electrophoresis Polyacrylamide Gel Deoxyribonucleases Type II Site-Specific Molecular Biology Nitrocellulose Polyacrylamide gel electrophoresis Plasmids |
| Zdroj: | Analytical Biochemistry. 134:265-268 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(83)90295-6 |
| Popis: | The incorporation into a 7% polyacrylamide gel of a nucleic acid-specific photochemical reagent 4,5′,8-trimethylpsoralen at a concentration of 1 mg/dl of acrylamide solution improves both the sensitivity and the efficiency of the transfer of plasmid DNA fragments from the gel onto nitrocellulose filters. This improvement allows detection by blot hybridization of DNA fragments as small as 57 bp long. |
| Databáze: | OpenAIRE |
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