Artemisia annua L. and photoresponse: from artemisinin accumulation, volatile profile and anatomical modifications to gene expression
| Autor: | Thália do Socorro Serra Gama, Andrea Furtado Macedo, Eliana Schwartz Tavares, Alessandra L. Valverde, Fábia Guimarães-Dias, Arthur Ladeira Macedo, Humberto R. Bizzo, Ellen Moura Lopes, Marcio Alves-Ferreira, Ana Cristina A. de Aguiar-Dias, Marcela Cristina de Moraes |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Light Artemisia annua Secondary Metabolism Plant Science Genetically modified crops Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Biosynthesis Gene Expression Regulation Plant parasitic diseases Gene expression Botany medicine Artemisinin Secondary metabolism Plant Proteins Epidermis (botany) General Medicine Trichomes biology.organism_classification Trichome Artemisinins Biosynthetic Pathways Plant Leaves 030104 developmental biology chemistry Agronomy and Crop Science 010606 plant biology & botany medicine.drug |
| Zdroj: | Plant cell reports. 39(1) |
| ISSN: | 1432-203X |
| Popis: | Blue and yellow light affected metabolism and the morphology. Blue and red promote the DOXP/MEP pathway. ADS gene expression was increased in plants cultivated under blue, promoting artemisinin content. Artemisinin-based combination therapies are the most effective treatment for highly lethal malaria. Artemisinin is produced in small quantities in the glandular trichomes of Artemisia annua L. Our aim was to evaluate the effect of light quality in A. annua cultivated in vitro under different light qualities, considering anatomical and morphological changes, the volatile composition, artemisinin content and the expression of two key enzymes for artemisinin biosynthesis. Yellow light is related to the increase in the number of glandular trichomes and this seemed to positively affect the molecular diversity in A. annua. Yellow light-stimulated glandular trichome frequency without triggered area enhancement, whereas blue light stimulated both parameters. Blue light enhanced the thickness of the leaf epidermis. The B-promoting effect was due to increased cell size and not to increased cell numbers. Green and yellow light positively influenced the volatile diversity in the plantlets. Nevertheless, blue and red light seemed to promote the DOXP/MEP pathway, while red light stimulates MVA pathway. Amorpha-4,11-diene synthase gene expression was significantly increased in plants cultivated under blue light, and not red light, promoting artemisinin content. Our results showed that light quality, more specifically blue and yellow light, positively affected secondary metabolism and the morphology of plantlets. It seemed that steps prior to the last one in the artemisinin biosynthesis pathway could be strongly influenced by blue light. Our work provides an alternative method to increase the amount of artemisinin production in A. annua without the use of transgenic plants, by the employment of blue light. |
| Databáze: | OpenAIRE |
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