The Effect of Creatine Kinase Inhibition on Contractile Properties of Human Resistance Arteries
| Autor: | Willem M. Ankum, Lizzy M. Brewster, Joseph F. Clark, Gert A. van Montfrans, Ed van Bavel, Fares A. Karamat, Zhila Taherzadeh |
|---|---|
| Přispěvatelé: | Vascular Medicine, Amsterdam Neuroscience - Neurovascular Disorders, Graduate School, Amsterdam Cardiovascular Sciences, Other Research, Obstetrics and Gynaecology, Biomedical Engineering and Physics, General Internal Medicine |
| Rok vydání: | 2015 |
| Předmět: |
Adult
medicine.medical_specialty Blood Pressure Vasodilation In Vitro Techniques 030204 cardiovascular system & hematology Contractility 03 medical and health sciences 0302 clinical medicine Internal medicine Internal Medicine medicine Humans Creatine Kinase biology business.industry Arteries Middle Aged medicine.anatomical_structure Blood pressure Endocrinology biology.protein Dinitrofluorobenzene Female Creatine kinase Sodium nitroprusside medicine.symptom business 030217 neurology & neurosurgery Vasoconstriction medicine.drug Artery Myograph |
| Zdroj: | American journal of hypertension, 29(2), 170-177. Oxford University Press |
| ISSN: | 1941-7225 0895-7061 |
| DOI: | 10.1093/ajh/hpv078 |
| Popis: | BACKGROUND Creatine kinase (CK) is a main predictor of blood pressure, and this is thought to largely depend on high resistance artery contractility. We previously reported an association between vascular contractility and CK in normotensive pregnancy, but pregnancy is a strong CK inducer, and data on human hypertension are lacking. Therefore, we further explored CK-dependency of vascular contractility outside the context of pregnancy in normotensive and hypertensive women. METHODS AND RESULTS Nineteen consecutive women, mean age 42 years (SE 1.3), mean systolic/diastolic blood pressure respectively 142.6 (SE 5.9)/85.6 (3.4) mm Hg (9 hypertensive), donated an omental fat sample during abdominal surgery. We compared vasodilation after the specific CK inhibitor 2,4-dinitro-1-fluorobenzene (DNFB; 10(-6) mol/l) to sodium nitroprusside (10(-6) mol/l) in isolated resistance arteries using a wire myograph. Additionally, we assessed predictors of vasoconstrictive force. DNFB reduced vascular contractility to 24.3% (SE 4.4), P < 0.001, compared to baseline. Sodium nitroprusside reduced contractility to 89.8% (SE 2.3). Maximum contractile force correlated with DNFB effect as a measure of CK (r = 0.8), and with vessel diameter (r = 0.7). The increase in contractile force was 16.5 mN [9.1-23.9] per unit DNFB effect in univariable and 10.35 mN [2.10-18.60] in multivariable regression analysis. CONCLUSION This study extends on our previous findings in pregnant normotensive women of CK-dependent microvascular contractility, indicating that CK contributes significantly to resistance artery contractility across human normotension and primary hypertension outside the context of pregnancy. Further studies should explore the effect of CK inhibitors on clinical blood pressure. |
| Databáze: | OpenAIRE |
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