Cervicovaginal Levels of Human β-Defensin 1, 2, 3, and 4 of Reproductive-Aged Women With Chlamydia trachomatis Infection
| Autor: | Larissa Doddi Marcolino, Aline Nascimento Bolpetti, Nathália Mayumi Noda-Nicolau, Camila Marconi, Larissa B. Bastos, Márcia Guimarães da Silva, Carolina Ferreira, Eliane Passarelli Vieira, Jossimara Polettini, Gabriel Vitor da Silva Pinto |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine medicine.medical_specialty beta-Defensins Adolescent 030106 microbiology Primary health care Chlamydia trachomatis Enzyme-Linked Immunosorbent Assay Cervix Uteri medicine.disease_cause Young Adult 03 medical and health sciences medicine Humans Statistical analysis Young adult Defensin Gynecology Chlamydia trachomatis infection business.industry Obstetrics and Gynecology General Medicine Chlamydia Infections Middle Aged 030104 developmental biology Vagina Neisseria gonorrhoeae Female Trichomonas vaginalis business Brazil |
| Zdroj: | Journal of Lower Genital Tract Disease. 21:189-192 |
| ISSN: | 1526-0976 1089-2591 |
| Popis: | OBJECTIVE This study included women attending primary health care units in Botucatu, Sao Paulo, Brazil, to assess the cervicovaginal levels of human β-defensin (hBD) 1, 2, 3, and 4 during Chlamydia trachomatis infection. PATIENTS AND METHODS Cervicovaginal samples were collected for Pap testing and assessing the presence of infection by C. trachomatis, human papillomavirus, Neisseria gonorrhoeae, and Trichomonas vaginalis. Vaginal smears were taken to evaluate local microbiota. Human β-defensin levels were determined using enzyme-linked immunosorbent assay in cervicovaginal fluid samples. Seventy-four women with normal vaginal microbiota and no evidence of infection were included in hBD quantification assays; 37 tested positive for C. trachomatis and 37 were negative. Statistical analysis was performed using Mann-Whitney U test. RESULTS Women positive for C. trachomatis had significantly lower cervicovaginal hBD-1, hBD-2, and hBD-3 compared with those who tested negative (hBD-1: 0 pg/mL [0-2.1] vs 1.6 pg/mL [0-2.4], p < .0001; hBD-2: 0 pg/mL [0-3.9] vs 0.61 pg/mL [0-8.9], p = .0097; and hBD-3: 0 pg/mL [0-4.3] vs 0.28 pg/mL [0-8.4], p = .0076). Human β-defensin 4 was not detected. CONCLUSIONS Lower levels of hBD-1, hBD-2, and hBD-3 in cervicovaginal fluid were detected in the presence of C. trachomatis infection. |
| Databáze: | OpenAIRE |
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