Distribution of free amino acids in streptozotocin-induced diabetic pregnant rats, their placentae and fetuses
| Autor: | C. E. Hendrich, A. D. Copeland, Susan P. Porterfield |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Blood Glucose
medicine.medical_specialty Aminoisobutyric Acids Endocrinology Diabetes and Metabolism medicine.medical_treatment Placenta Clinical Biochemistry Biology Biochemistry Diabetes Mellitus Experimental Endocrinology Fetus Pregnancy Diabetes mellitus Internal medicine medicine Animals Insulin Amino Acids Pancreatic hormone chemistry.chemical_classification Biochemistry (medical) Rats Inbred Strains General Medicine medicine.disease Streptozotocin Amino acid Rats medicine.anatomical_structure chemistry Gestation Female medicine.drug |
| Zdroj: | Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. 22(2) |
| ISSN: | 0018-5043 |
| Popis: | Amino acid levels in the non-pregnant streptozotocin (STZ)-induced diabetic rat have been shown to be abnormal. Our preliminary studies showed that placental transport, fetal serum levels and tissue uptake of the non-metabolizable amino acid alpha-amino isobutyric acid (AIB) were decreased in STZ-diabetic pregnant rats. In the present experiments, amino acid concentrations were measured in maternal (MS) and fetal (FS) sera and placentae (PL) by high performance liquid chromatography (HPLC) after triple extraction in 80% ethanol. Control (C), STZ-diabetic (D) and insulin-treated diabetic (DI) animals were studied at 22 days gestation. Pregnant diabetic rats had low serum levels of Gln, Lys, and Ser and insulin treatment corrected Gln and Ser but not Lys levels. Branched-chain amino acids did not show the large elevation characteristic of the non-pregnant diabetic rat. Placental levels of Tau, Gln, HPr, Thr and Lys were depressed in the diabetic animals and insulin treatment only partially improved these amino acid profiles. Placental amino acid levels did not always reflect maternal serum levels. Serum levels of most amino acids were lower in the fetus of the diabetic rat than in the fetus of the control rat. The notable exception was Ala which was higher in the fetuses of the diabetic animals. Insulin treatment of the mother did not correct many of the fetal amino acid levels even though maternal and fetal serum glucose levels at the time of autopsy were normal. The ability to maintain normal serum levels of many amino acids is impaired in the fetus of the diabetic rat.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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