Antitubercular Activity and Inhibitory Effect on Epstein-Barr Virus Activation of Sterols and Polyisoprenepolyols from an Edible Mushroom, Hypsizigus marmoreus
| Autor: | Yumiko Kimura, Ken Yasukawa, Masaaki Tagata, Toshihiro Akihisa, Tsunetomo Matsuzawa, Scott G. Franzblau, Motohiko Ukiya, Harukuni Tokuda, Koichi Metori, Takashi Suzuki |
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| Rok vydání: | 2005 |
| Předmět: |
Herpesvirus 4
Human Antitubercular Agents Pharmaceutical Science medicine.disease_cause Antiviral Agents Virus Microbiology Mycobacterium tuberculosis Minimum inhibitory concentration Hemiterpenes Cell Line Tumor Pentanes Butadienes medicine Humans Inhibitory effect Pharmacology Strain (chemistry) biology General Medicine biology.organism_classification Epstein–Barr virus Raji cell Edible mushroom Sterols Agaricales |
| Zdroj: | Biological and Pharmaceutical Bulletin. 28:1117-1119 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.28.1117 |
| Popis: | Seven sterols (1-7) and eight polyisoprenepolyols (8-15), isolated from the non-saponifiable lipid fraction of the dichloromethane extract of an edible mushroom, Hypsizigus marmoreus (Buna-shimeji), were tested for their antitubercular activity against Mycobacterium tuberculosis strain H37Rv using the Microplate Alamar Blue Assay (MABA). Six sterols (2-7) and two polyisoprenepolyols (8, 12) showed a minimum inhibitory concentration (MIC) in the range of 1-51 microg/ml, while the others (1, 9-11, 13-15) were inactive (MIC>128 microg/ml). The seven sterols (1-7) and three polyisoprenepolyols (8, 10, 12) were further evaluated for their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells. Sterols 6 and 7 showed potent inhibitory effects while preserving the high viability of Raji cells. |
| Databáze: | OpenAIRE |
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