An in vitro system for assessing the sensitivity of Plasmodium vivax to chloroquine
Autor: | Jeeraphat Sirichaisinthop, Harald Noedl, Oumaporn Tasanor, Walther H. Wernsdorfer, Kesara Na-Bangchang, Kanungnit Congpuong |
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Rok vydání: | 2002 |
Předmět: |
biology
Inoculation Veterinary (miscellaneous) Plasmodium vivax Chloroquine biology.organism_classification Thailand Molecular biology In vitro Apicomplexa Antimalarials Infectious Diseases Parasitic Sensitivity Tests Insect Science Immunology medicine Parasite hosting Animals Parasitology Incubation Cells Cultured medicine.drug EC50 |
Zdroj: | Acta tropica. 83(1) |
ISSN: | 0001-706X |
Popis: | Following earlier observations on short-term culture of Plasmodium iax, an in vitro test system has been developed for assessing the parasite’s sensitivity to chloroquine. Fresh isolates with predominantly young trophozoites are diluted 1:19 with a (v/v=1/1) mixture of RPMI 1640 and Waymouth medium. The blood-medium mixture (BMM) is inoculated into the predosed microtitre plates before incubation in a candle jar. Incubation for 30 or 42 h yielded the best results. Incubation for 18 or 24 h was generally insufficient for an adequate development of the parasites. The reading of the test is based on stage-specific differential counts in the Giemsa-stained pre-incubation and post-incubation thick films, the evaluation on log-probit analysis of drug-related inhibition of parasite development. The test system has been evaluated on 200 fresh P. iax isolates in an area with satisfactory clinical-parasitological response to chloroquine. At 30 or 42 h incubation 121 isolates (61.5%) showed adequate control growth and yielded valid sensitivity tests. Complete inhibition of parasite development occurred within the concentration range of 40–1280 nM. The mean EC50 for 30 h of incubation was 50.3 nM, as compared to 49.7 nM with 42 h of incubation. The geometric mean cut-off concentration of parasite development was 488 nM with 30 h of incubation as against 470 nM with 42 h of incubation. © 2002 Elsevier Science Ireland Ltd. All rights reserved. |
Databáze: | OpenAIRE |
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