Indirect enzyme-linked immunosorbent assay for detection of Brucella melitensis-specific antibodies in goat milk
| Autor: | N. D. Funk, Lorraine J. Hoffman, Louisa B. Tabatabai, Philip H. Elzer, Sue D. Hagius, B. M. Martin |
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| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Veterinary medicine Population Brucellaceae Enzyme-Linked Immunosorbent Assay Brucella Sensitivity and Specificity Brucellosis Clinical Veterinary Microbiology Antigen Antibody Specificity medicine Brucella melitensis Animals education education.field_of_study Antigens Bacterial Goat Diseases biology Goats biology.organism_classification medicine.disease Virology Antibodies Bacterial Milk Herd Female |
| Zdroj: | Journal of clinical microbiology. 43(2) |
| ISSN: | 0095-1137 |
| Popis: | Brucella melitensisis the cause of brucellosis in sheep and goats, which often results in abortion. Few cases ofB. melitensisinfection in goats have occurred in the United States over the last 25 years. However, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not introduced into the U.S. goat population. The objective of this study was to develop a sensitive and specific indirect enzyme-linked immunosorbent assay (iELISA) for the detection ofB. melitensis-specific antibodies in goat milk.Brucellasalt-extractable protein extract was employed as an antigen, and a horseradish peroxidase-labeled polyclonal anti-goat antibody was used as an anti-species conjugate. Thirteen of 13 (100%) individual infected goat milk samples tested positive and 134 of 134 (100%) uninfected bulk milk samples tested negative by the developed iELISA. Three positive milk samples with high, medium, and low absorbance values were used to simulate one positive animal in an otherwise negative herd. By this estimation, one high-titer animal could be detected in a herd of >1,600 animals. Detection estimates for medium- and low-titer animals were one positive animal per herd of B. melitensis-specific antibodies in goat milk. |
| Databáze: | OpenAIRE |
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