Wnt-11 and Fz7 reduce cell adhesion in convergent extension by sequestration of PAPC and C-cadherin
| Autor: | Corinna D. Berger, Herbert Steinbeisser, Bianca Kraft, Veronika Wallkamm, Doris Wedlich |
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| Rok vydání: | 2012 |
| Předmět: |
animal structures
Embryo Nonmammalian media_common.quotation_subject Xenopus Down-Regulation Protocadherin Xenopus Proteins Biology Article Receptors G-Protein-Coupled Xenopus laevis Cell Adhesion Animals Cell adhesion Internalization Research Articles media_common Convergent extension Cadherin Gastrulation Wnt signaling pathway Cell Biology Cell sorting Cadherins biology.organism_classification Protocadherins Cell biology Wnt Proteins Multiprotein Complexes Oocytes Female Protein Multimerization |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.201110076 |
| Popis: | Wnt-11/frizzled-7 reduces the lateral clustering of C-cadherin by capturing the protocadherin PAPC and C-cadherin into distinct adhesion-modulating complexes. Wnt-11/planar cell polarity signaling polarizes mesodermal cells undergoing convergent extension during Xenopus laevis gastrulation. These shape changes associated with lateral intercalation behavior require a dynamic modulation of cell adhesion. In this paper, we report that Wnt-11/frizzled-7 (Fz7) controls cell adhesion by forming separate adhesion-modulating complexes (AMCs) with the paraxial protocadherin (PAPC; denoted as AMCP) and C-cadherin (denoted as AMCC) via distinct Fz7 interaction domains. When PAPC was part of a Wnt-11–Fz7 complex, its Dynamin1- and clathrin-dependent internalization was blocked. This membrane stabilization of AMCP (Fz7/PAPC) by Wnt-11 prevented C-cadherin clustering, resulting in reduced cell adhesion and modified cell sorting activity. Importantly, Wnt-11 did not influence C-cadherin internalization; instead, it promoted the formation of AMCC (Fz7/Cadherin), which competed with cis-dimerization of C-cadherin. Because PAPC and C-cadherin did not directly interact and did not form a joint complex with Fz7, we suggest that Wnt-11 triggers the formation of two distinct complexes, AMCC and AMCP, that act in parallel to reduce cell adhesion by hampering lateral clustering of C-cadherin. |
| Databáze: | OpenAIRE |
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