Extraction of the antioxidant phytocomplex from wine-making by-products and sustainable loading in phospholipid vesicles specifically tailored for skin protection
| Autor: | Maria Letizia Manca, José Luis Pedraz, Antonio Segura-Carretero, Gianluigi Bacchetta, M Perra, Jesús Lozano-Sánchez, Francisco Javier Leyva-Jiménez, Maria Manconi, Aldo Muntoni, Giorgia De Gioannis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Phytochemicals Ethanol-water extraction Wine RM1-950 Antioxidants Mice chemistry.chemical_compound Skin delivery medicine Maceration (wine) Phospholipid vesicles Animals Vitis Food science Gallic acid Particle Size Phospholipids Skin Pharmacology Plant Extracts Vesicle Pomace Catechin 3T3 Cells Hydrogen Peroxide General Medicine Fibroblasts Grape pomace Oxidative Stress chemistry Liposomes Therapeutics. Pharmacology Quercetin |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 142, Iss, Pp 111959-(2021) Digibug. Repositorio Institucional de la Universidad de Granada instname |
| Popis: | The authors thank for technical and human support provided by TEM measurements carried out in the SGIker Polymer Characterization (UPV/EHU/ERDF, EU) . The authors also thank: MIUR and PON R&I for financing the PhD grant; BESTMEDGRAPE - ENI CBC Med project for providing analytical equipment and materials; Cantina Santadi for providing Carignano grape pomace. The authors acknowledge the CeSAR (Centro Servizi d'Ateneo per la Ricerca) of the University of Cagliari, Italy for the granulometry experiments performed with Mal-vern Mastersizer 3000. The present study is aimed at valorizing grape pomace, one of the most abundant winery-making by-products of the Mediterranean area, through the extraction of the main bioactive compounds from the skin of grape pomace and using them to manufacture innovative nanoformulations capable of both avoiding skin damages and promoting skincare. The phytochemicals were recovered through maceration in hydroethanolic solution. Catechin, quercetin, fisetin and gallic acid, which are known for their antioxidant power, were detected as the main compounds of the extract. Liposomes and phospholipid vesicles modified with glycerol or Montanov 82 (R) or a combination of both, were used as carriers for the extract. The vesicles were small (-183 nm), slightly polydispersed (PI >= 0.28), and highly negatively charged (--50 mV). The extract was loaded in high amounts in all vesicles (-100%) irrespective of their composition. The antioxidant activity of the extract, measured by using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, was 84 +/- 1%, and slightly increased when loaded into the vesicles (-89%, P < 0.05). The grape pomace extract loaded vesicles were highly biocompatible and able to protect fibroblasts (3T3) from the oxidative stress induced by hydrogen peroxide. Ministry of Education, Universities and Research (MIUR) PON RI |
| Databáze: | OpenAIRE |
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