Characterization of carbapenem-resistant gram-negative bacterial isolates from Nigeria by whole genome sequencing
| Autor: | Jack Sumner, Caitlin M. dela Cruz, Isabella A. Tickler, Victoria M. Le, Scott Dewell, Fred C. Tenover, Shuwaram A. Shettima |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_treatment 030106 microbiology Nigeria Microbial Sensitivity Tests Biology medicine.disease_cause beta-Lactam Resistance beta-Lactamases Microbiology 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Gram-Negative Bacteria medicine Humans 030212 general & internal medicine Escherichia coli Whole genome sequencing Pseudomonas aeruginosa Providencia rettgeri General Medicine biology.organism_classification Anti-Bacterial Agents Stenotrophomonas maltophilia Infectious Diseases Carbapenems Beta-lactamase Gram-Negative Bacterial Infections Enterobacter cloacae Bacteria Genome Bacterial |
| Zdroj: | Diagnostic microbiology and infectious disease. 101(1) |
| ISSN: | 1879-0070 |
| Popis: | This study characterized the mechanisms of carbapenem resistance in gram-negative bacteria isolated from patients in Yola, Nigeria. Whole genome sequencing (WGS) was performed on 66 isolates previously identified phenotypically as carbapenem-non-susceptible. The patterns of beta-lactamase resistance genes identified were primarily species-specific. However, blaNDM-7 and blaCMY-4 were detected in all Escherichia coli and most Providencia rettgeri isolates; blaNDM-7 was also detected in 1 Enterobacter cloacae. The E. coli and E. cloacae isolates also shared blaOXA-1, while blaOXA-10 was found in all P. rettgeri, one Pseudomonas aeruginosa and 1 E. coli. Except for Stenotrophomonas maltophilia isolates, which only contained blaL1, most species carried multiple beta-lactamase genes, including those encoding extended-spectrum beta-lactamases, AmpC and OXA in addition to a carbapenemase gene. Carbapenemase genes were either class B or class D beta-lactamases. No carbapenemase gene was detected by WGS in 13.6% of isolates. |
| Databáze: | OpenAIRE |
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