Detection of ebaf, a novel human gene of the transforming growth factor beta superfamily association of gene expression with endometrial bleeding
| Autor: | Siamak Tabibzadeh, Kothapalli R, S Q Wu, N Chegini, Buyuksal I |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Adult
medicine.medical_specialty media_common.quotation_subject Molecular Sequence Data Endometriosis In situ hybridization Endometrium Polymerase Chain Reaction Transforming Growth Factor beta Internal medicine Gene expression medicine Humans Amino Acid Sequence Menorrhagia Menstrual Cycle Menstruation Disturbances Menstrual cycle DNA Primers media_common Base Sequence Leiomyoma Sequence Homology Amino Acid biology medicine.diagnostic_test Chromosome Mapping Transforming growth factor beta superfamily General Medicine Transforming growth factor beta medicine.disease Endocrinology medicine.anatomical_structure Chromosomes Human Pair 1 Multigene Family Uterine Neoplasms biology.protein Cancer research Female Research Article Fluorescence in situ hybridization |
| Zdroj: | Journal of Clinical Investigation. 99:2342-2350 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci119415 |
| Popis: | Human endometrium is unique since it is the only tissue in the body that bleeds at regular intervals. In addition, abnormal endometrial bleeding is one of the most common manifestations of gynecological diseases, and is a prime indication for hysterectomy. Here, we report on a novel human gene, endometrial bleeding associated factor (ebaf), whose strong expression in endometrium was associated with abnormal endometrial bleeding. In normal human endometrium, this gene was transiently expressed before and during menstrual bleeding. In situ hybridization showed that the mRNA of ebaf was expressed in the stroma without any significant mRNA expression in the endometrial glands or endothelial cells. The predicted protein sequence of ebaf showed homology with and structural features of the members of TGF-beta superfamily. Fluorescence in situ hybridization showed that the ebaf gene is located on human chromosome 1 at band q42.1. Thus, ebaf is a novel member of the TGF-beta superfamily and an endometrial tissue factor whose expression is associated with normal menstrual and abnormal endometrial bleeding. |
| Databáze: | OpenAIRE |
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