Single gene alteration of plasma and mitochondrial membrane function in Saccharomyces cerevisiae
| Autor: | G. H. Rank, A. J. Robertson, J. H. Gerlach |
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| Rok vydání: | 1977 |
| Předmět: |
ATPase
Saccharomyces cerevisiae Mutant chemistry.chemical_compound Ethidium Genetics medicine Selection Genetic Inner mitochondrial membrane Molecular Biology Adenosine Triphosphatases biology Chloramphenicol Cell Membrane Drug Resistance Microbial biology.organism_classification Molecular biology Mitochondria Membrane Genes Biochemistry chemistry Mutation biology.protein Energy source Ethidium bromide medicine.drug |
| Zdroj: | Molecular and General Genetics MGG. 152:13-18 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00264934 |
| Popis: | Some physiological properties of a multiple-drug-resistant mutant with a permeability barrier to chloramphenicol and its isogenic parental strain were compared. The ATPase specific activity of plasma and mitochondrial membranes isolated from the mutant strain was approximately 20% lower (P less than 0.001, Tables 1 and 2) than that of membranes isolated from the isogenic parental strain. Additional evidence of altered mitochondrial function was: (i) the enhanced growth of the parental strain was eliminted by the [rho-] state (Table 3); (ii) the mutant strain had a greater resistance to petite induction by ethidium bromide (Table 4); (iii) the mutant strain was unable to use a nonfermentable energy source for respiratory adaptation (Table 5). It is proposed that a single gene mutation has resulted in an alteration of some physiological properties of the plasma and mitochondrial membranes. |
| Databáze: | OpenAIRE |
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