Direct observation of stepwise movement of a synthetic molecular transporter
| Autor: | Andrew J. Turberfield, Yousuke Katsuda, Hiroshi Sugiyama, Shelley F. J. Wickham, Jonathan Bath, Kumi Hidaka, Masayuki Endo |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Models
Molecular Materials science Surface Properties Movement Restriction Mapping Biomedical Engineering DNA Single-Stranded Observation Bioengineering Nanotechnology Microscopy Atomic Force Motion General Materials Science A-DNA Electrical and Electronic Engineering Base Pairing Base Sequence Movement (music) Atomic force microscopy Track (disk drive) Molecular machines and motors Direct observation Biological Transport Transporter DNA walker Nanobiotechnology DNA Condensed Matter Physics Atomic and Molecular Physics and Optics Nanostructures Feasibility Studies Nucleic Acid Conformation Biological system Constant (mathematics) |
| Zdroj: | Nature nanotechnology. 6(3):166-169 |
| ISSN: | 1748-3395 |
| Popis: | Controlled motion at the nanoscale can be achieved by using Watson-Crick base-pairing to direct the assembly and operation of a molecular transport system consisting of a track, a motor and fuel, all made from DNA. Here, we assemble a 100-nm-long DNA track on a two-dimensional scaffold, and show that a DNA motor loaded at one end of the track moves autonomously and at a constant average speed along the full length of the track, a journey comprising 16 consecutive steps for the motor. Real-time atomic force microscopy allows direct observation of individual steps of a single motor, revealing mechanistic details of its operation. This precisely controlled, long-range transport could lead to the development of systems that could be programmed and routed by instructions encoded in the nucleotide sequences of the track and motor. Such systems might be used to create molecular assembly lines modelled on the ribosome. |
| Databáze: | OpenAIRE |
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