Translational Accuracy during Exponential, Postdiauxic, and Stationary Growth Phases in Saccharomyces cerevisiae
| Autor: | Samia N. Ben Salem, Lifeng Chen, Bing Zhao, Guillaume Stahl, Philip J. Farabaugh |
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| Rok vydání: | 2004 |
| Předmět: |
Messenger RNA
Translational frameshift Ethanol Retroelements Saccharomyces cerevisiae Frameshifting Ribosomal RNA General Medicine RNA Transfer Amino Acyl Biology biology.organism_classification Microbiology Article Yeast Exponential function Glucose Biochemistry Protein Biosynthesis Transfer RNA Protein biosynthesis Biophysics sense organs Codon Molecular Biology |
| Zdroj: | Eukaryotic Cell. 3:331-338 |
| ISSN: | 1535-9786 1535-9778 |
| Popis: | When the yeast Saccharomyces cerevisiae shifts from rapid growth on glucose to slow growth on ethanol, it undergoes profound changes in cellular metabolism, including the destruction of most of the translational machinery. We have examined the effect of this metabolic change, termed the diauxic shift, on the frequency of translational errors. Recoding sites are mRNA sequences that increase the frequency of translational errors, providing a convenient reporter of translational accuracy. We found that the diauxic shift causes no overall change in translational accuracy but does cause a strong reduction in the frequency of one type of programmed error: Ty +1 frameshifting. Genetic data suggest that this effect may be due to changes in the relative amounts of tRNA participating in translation elongation. We discuss possible implications for expression strategies that use recoding. |
| Databáze: | OpenAIRE |
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