Radiotherapy Suppresses Bone Cancer Pain through Inhibiting Activation of cAMP Signaling in Rat Dorsal Root Ganglion and Spinal Cord
| Autor: | Rubing Zhou, Yanbin Dong, Guiqin Zhu, Aixing Yang, Zhong Xie, Xue-Jun Song, Xueming He, Jianhua Ma, Ping Zhao |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Article Subject medicine.medical_treatment Immunology Bone Neoplasms Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Dorsal root ganglion CAMP signaling Internal medicine Ganglia Spinal lcsh:Pathology medicine Animals Messenger RNA business.industry Bone cancer Cell Biology Cancer Pain medicine.disease Spinal cord Rats Radiation therapy 030104 developmental biology medicine.anatomical_structure Endocrinology Spinal Cord Anesthesia Female Signal transduction business Cancer pain 030217 neurology & neurosurgery lcsh:RB1-214 Signal Transduction Research Article |
| Zdroj: | Mediators of Inflammation Mediators of Inflammation, Vol 2016 (2016) |
| ISSN: | 1466-1861 |
| Popis: | Radiotherapy is one of the major clinical approaches for treatment of bone cancer pain. Activation of cAMP-PKA signaling pathway plays important roles in bone cancer pain. Here, we examined the effects of radiotherapy on bone cancer pain and accompanying abnormal activation of cAMP-PKA signaling. Female Sprague-Dawley rats were used and received tumor cell implantation (TCI) in rat tibia (TCI cancer pain model). Some of the rats that previously received TCI treatment were treated with X-ray radiation (radiotherapy). Thermal hyperalgesia and mechanical allodynia were measured and used for evaluating level of pain caused by TCI treatment. PKA mRNA expression in dorsal root ganglion (DRG) was detected by RT-PCR. Concentrations of cAMP, IL-1β, and TNF-αas well as PKA activity in DRG and the spinal cord were measured by ELISA. The results showed that radiotherapy significantly suppressed TCI-induced thermal hyperalgesia and mechanical allodynia. The level of PKA mRNA in DRG, cAMP concentration and PKA activity in DRG and in the spinal cord, and concentrations of IL-1βand TNF-αin the spinal cord were significantly reduced by radiotherapy. In addition, radiotherapy also reduced TCI-induced bone loss. These findings suggest that radiotherapy may suppress bone cancer pain through inhibition of activation of cAMP-PKA signaling pathway in DRG and the spinal cord. |
| Databáze: | OpenAIRE |
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