3D visualization of HIV transfer at the virological synapse between dendritic cells and T cells
| Autor: | Bradley C. Lowekamp, Jacob D. Estes, Jing Fu, Charles M. Trubey, Kedar Narayan, Jeffrey D. Lifson, Sriram Subramaniam, Douglas K. Schneider, Gordon Ruthel, Richard L. Felts, Dan Shi, Kunio Nagashima, Julian W. Bess, Lisa M. Hartnell, Sina Bavari, Donald Bliss |
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| Rok vydání: | 2010 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cell signaling Multidisciplinary T-Lymphocytes Virion Antigen-Presenting Cells HIV Dendritic cell Cell Communication Dendritic Cells Biology Biological Sciences Virus Cell biology Synapse Electron tomography Microscopy Electron Transmission Microscopy Fluorescence Viral entry Host-Pathogen Interactions Extracellular Microscopy Electron Scanning Humans Antigen-presenting cell |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 107(30) |
| ISSN: | 1091-6490 |
| Popis: | The efficiency of HIV infection is greatly enhanced when the virus is delivered at conjugates between CD4 + T cells and virus-bearing antigen-presenting cells such as macrophages or dendritic cells via specialized structures known as virological synapses. Using ion abrasion SEM, electron tomography, and superresolution light microscopy, we have analyzed the spatial architecture of cell-cell contacts and distribution of HIV virions at virological synapses formed between mature dendritic cells and T cells. We demonstrate the striking envelopment of T cells by sheet-like membrane extensions derived from mature dendritic cells, resulting in a shielded region for formation of virological synapses. Within the synapse, filopodial extensions emanating from CD4 + T cells make contact with HIV virions sequestered deep within a 3D network of surface-accessible compartments in the dendritic cell. Viruses are detected at the membrane surfaces of both dendritic cells and T cells, but virions are not released passively at the synapse; instead, virus transfer requires the engagement of T-cell CD4 receptors. The relative seclusion of T cells from the extracellular milieu, the burial of the site of HIV transfer, and the receptor-dependent initiation of virion transfer by T cells highlight unique aspects of cell-cell HIV transmission. |
| Databáze: | OpenAIRE |
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