The influence of female sex hormones on lung inflammation after brain death ‐ an experimental study

Autor: Fernanda Yamamoto Ricardo-da-Silva, Roberto Armstrong-Jr, Yanira Riffo-Vasquez, Cristiano de Jesus Correia, Luiz Felipe P. Moreira, Sueli Gomes Ferreira, Ana Cristina Breithaupt-Faloppa, Marina Vidal-Dos-Santos, Ana Luisa de Oliveira Bonnano Abib
Rok vydání: 2019
Předmět:
Zdroj: Transplant International. 33:279-287
ISSN: 1432-2277
0934-0874
Popis: Organ donor's age negatively influences graft survival of organs, increasing risk of complications. Aging occurs in both men and women; however, the menopause marks a decrease in sex hormones and a sudden increase in the process of vascular aging. We investigated sex hormones' influence on the lung inflammatory process induced by BD in female rats. Wistar rats were grouped as: female rats from high estradiol to heat period (non-OVx) and ovariectomized (OVx) female rats. Ovariectomy was carried out 10 days before BD. BD was induced using intracranial balloon rapid inflation. Serum hormones and inflammatory mediators were quantified, leukocytes and platelets counted and lung samples were collected for RT-PCR, immunohistochemical, and histological analysis. Female sex hormones and corticosterone were reduced 6 h after BD in non-OVx group. The infiltration of leukocytes in female non-OVx lungs was higher compared to OVx. G-CSF, VEGF, and CINC-1 were found increased in non-OVx group serum in comparison to OVx. Lung mediators were increased in non-OVx rats compared to controls. The acute reduction of sex hormones induced by BD appears to have a worse effect on lung inflammation than a reduction that has happened over a prolonged period of time, allowing a physiological adaptation prior to BD.
Databáze: OpenAIRE
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