Apparent successful mesothelial cell transplantation hampered by peritoneal activation
| Autor: | Robert H. J. Beelen, L H Hekking, Eelco D. Keuning, Bas A. J. Driesprong, Dirk R. De Waart, Machteld M. Zweers, Jacob van den Born |
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| Přispěvatelé: | Amsterdam Gastroenterology Endocrinology Metabolism, Tytgat Institute for Liver and Intestinal Research, Groningen Kidney Center (GKC), Groningen Institute for Organ Transplantation (GIOT) |
| Rok vydání: | 2005 |
| Předmět: |
Nephrology
Male medicine.medical_specialty Pathology medicine.medical_treatment Cells Wistar Peritonitis Lymphocytes/immunology Peritoneal inflammation Epithelial Cells/cytology Epithelium Peritoneal dialysis Peritoneum Internal medicine medicine Animals Lymphocytes Mast Cells Rats Wistar peritonitis Cells Cultured mesothelial cells Cultured business.industry Epithelial Cells medicine.disease Omentum/cytology Rats Transplantation medicine.anatomical_structure Thioglycolates Peritoneum/cytology Mast Cells/immunology business Omentum Peritonitis/chemically induced Mesothelial Cell transplantation |
| Zdroj: | Kidney international, 68(5), 2362-2367. Nature Publishing Group Kidney International, 68(5), 2362-2367. ELSEVIER SCIENCE INC |
| ISSN: | 0085-2538 |
| DOI: | 10.1111/j.1523-1755.2005.00698.x |
| Popis: | Apparent successful mesothelial cell transplantation hampered by peritoneal activation.BackgroundMesothelial cell transplantation has been suggested to improve mesothelial repair after surgery, recurrent peritonitis and peritoneal dialysis.MethodsIn this study we evaluated mesothelial cell transplantation during the resolution phase of experimentally thioglycollate-induced peritonitis in rats. To this end 4 × 106 DiO-labeled autologous mesothelial cells were transplanted 1 week after peritonitis induction. Peritoneal inflammation and permeability characteristics were evaluated after another week.ResultsMesothelial cell transplantation after peritonitis resulted in incorporation of these cells in the parietal mesothelial lining, leading to an acute transient submesothelial thickening which was not seen in transplanted animals without prior peritonitis induction. Long-term functioning of these repopulated mesothelial cells leaded to peritoneal activation as evidenced by a ∼twofold increase in peritoneal lymphocytes (P < 0.01) and omental mast cell counts (P < 0.05), accompanied by the induction of inflammation markers monocyte chemoattractant protein-1 (MCP-1) (P < 0.01) and hyaluronan (P < 0.01) in the transplanted peritonitis group, but not in rats with peritonitis without mesothelial cell transplantation or in control rats without mesothelial cell transplantation (all four parameters P < 0.01). In addition, trapping of transplanted mesothelial cells in the milky spots of omental tissue and lymphatic stomata of the diaphragm both in control and thioglycollate rats seems to increase microvascular permeability, reflected by apparent increased diffusion rates of small solutes and proteins.ConclusionAltogether, our data underscore the importance of controlling peritoneal (patho)physiology and function in mesothelial transplantation protocols. |
| Databáze: | OpenAIRE |
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