Chromosome delineation induced by a combined potassium permanganate-sodium bisulfite treatment
| Autor: | A. Novello, M. E. Drets |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Cytological Procedure
Cytological Techniques Biology Tritium Giemsa stain Chromosomes chemistry.chemical_compound Mice Potassium Permanganate Bone Marrow Genetics Animals Humans Sulfites Lymphocytes Genetics (clinical) Cells Cultured Chromosome Molecular biology Cytophotometry Chromatin chemistry Sodium bisulfite Autoradiography Interphase DNA Thymidine |
| Zdroj: | Chromosoma. 78(3) |
| ISSN: | 0009-5915 |
| Popis: | A cytological procedure for in vitro chromosome delineation has been studied using human and mouse (Mus musculus) chromosomes. This method, consisting of slide incubation in KMnO4 at 0--5 degrees C for 24 h followed by a short exposure to NaHSO3 (1--3 min) and Giemsa staining, induces extraction of chromatin from human and mouse interphase nuclei and chromosomes. Autoradiography after 3H-ThD incorporation in vitro and cytophotometry confirmed that DNA is removed. Well contour-delineated and non-distorted chromosomes are observed in both species allowing the identification of all human chromosome groups. Contour chromosome delineation and its relationship to chromosome organization is briefly discussed. |
| Databáze: | OpenAIRE |
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