| Autor: |
Buß, Oliver, Sarah-Marie Dold, Obermeier, Pascal, Litty, Dennis, Muller, Delphine, Grüninger, Jens, Rudat, Jens |
| Rok vydání: |
2018 |
| DOI: |
10.6084/m9.figshare.7120721.v1 |
| Popis: |
Additional file 1: Figure S1. Acetophenone (AP) content during fermentation process in comparison to (S)-β-PA concentration. AP concentration rises inversely proportional to (S)-β-PA degradation and decreases after (S)-β-PA depletion. a) BS115 fermentation b) PsJN fermentation. Figure S2. Extracellular capsule built by BS115 during fermentation after depletion of (S)-β-PA. The capsule of BS115 was visualized by negative contrasting with Chinese ink. Figure S3. Transaminase activity of cell free lysate of BS115 and PsJN. In red triangles: (R)-β-PA; in green triangles (S)-β-PA. The reaction was performed at 30 °C in reaction mixture (see also section 2.6) of 12 mM of rac- β-PA using α-ketoglutarate as amino acceptor. The reaction and sampling time was chosen to depict fast reactions as well as possible long-term effects. Figure S4. pH profile during fermentation of BS115 and PsJN in 1.5 L bioreactor systems using minimal medium. In contrast to PsJN, BS115 showed a stabilization of the pH value between 20 and 40 h. Figure S5. Chiral separation of β-PA using IBLC-OPA pre-column derivatization and reversed phase HPLC according to Brucher et al. (2010a).The retention time of the (R)-enantiomer is 3.7 min, of the (S)-enantiomer 4.8 min. Figure S6. Relative transaminase activity of BS115 in regard to pH. The reactions were performed with 2.5 mM of racemic β-phenylalanine using 0.5 mg/mL of protein at 30 °C. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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